International Journal of Marine Science, 2017, Vol.7, No.24, 229-246
230
B-Explain the histopathological changes altered by
Amyloodiniosis
.
C-Statistical analysis was made with the computer program (
Statistical Package for Social Sciences
)
SPSS
.
Differences among groups were assessed by means of analysis of variance (ANOVA). Subsequently, significance of
differences between values was tested with the LSD post-hoc test (least significant difference test) to detect
particular differences between groups. Values are represented as mean ± standard error (mean ± S.E.M).
Significance indicated in figures and tables by an asterisk (*) was taken as
p
<0.05. Statistical analysis on data that
that obtained from:
1-Investigate the occurrence of
Amyloodiniosis
in EuropeanSeabass
Dicentrarchus labrax
.
2-Study the ecological factors affects
Amyloodiniosis
.
3-Treatement measures using for
Amyloodiniosis.
1 Materials and Methods
1.1 Fish
1.1.1 Fish used for natural examination
A total number of 1065 European Sea bass
(Dicentrarchus labrax)
were obtained from different marine fish farms
in Egypt and examined for
Amyloodinium ocellatum.
1.1.2 Fish used for experimental examination
A total number of 120
D. labrax
fingerlings were obtained and acclimatized in fiberglass aquaria for conduction
of treatment trial of
Amyloodinium ocellatum
infection.
1.1.3 Aquaria and concrete pond
Dicentrarchus labrax
used for natural examinations, were held in Glass aquaria (40×50×60 cm).
Dicentrarchus labrax
fingerlings used for conduction of treatment trial of
Amyloodinium ocellatum
infection,
were held in fiberglass tanks (1000 L).
The used fiberglass tanks and Glass aquaria were supplied with fresh seawater and aerated through air blower.
Natural seawater should be with water temperature ranged at (25 ±3°C), Dissolved Oxygen D.O 5±3 mg/L, pH
7.4-8.8, salinity at 12±3‰ salinity and supplied with air systems.
1.2 Fish sampling
European
D. labrax
were sampled fromApril 2015 to April 2016. Freshly dead or morbid fish were taken and kept
in ice boxes and transported as soon as possible to fish diseases laboratory in the National institute of oceanography
and fisheries.
1.3 Water sampling
They were collected in glass or plastic and transported to the laboratory in insulated coolers and examined within
(24-48 h). They include water temperature, dissolved oxygen, pH, ammonia (NH3/NH4
+
) and salinity, water
temperature, dissolved oxygen and salinity were measured at collection sites. All water quality parameters were
analyzed according to
(APHA, 2005).
1.4 Laboratory diagnosis of
Amyloodiniosis
1.4.1 Clinical investigation and Post Mortem (PM) examination
It was performed according to Noga (2010). Fish samples with various clinical symptoms like excessive mucous,
erosions on the fins, deformity on vertebral column, pale to brownish gills were brought to the laboratory.
1.4.2 Parasitological examination
Macroscopic examination
Skin surface, fins and gills were examined by naked eyes and with the help of dissecting microscope for any
attached parasites, lesions or external changes.
