International Journal of Marine Science, 2017, Vol.7, No.31, 297-307
307
Table 2 Data on average test results of activity of varying concentrations crude extract of
H. gracillis
to test bacteria E
. coli,
S.dysenteriae,
and
B. subtillis
Sampel uji
Konsentrasi
(ppm)
Diameter Zona Bening (mm)
E. coli
S. dysenteriae
B.subtilis
Ekstrak Kasar etil asetat
200.000
9,47
11,09
8,84
100.000
0,0
0,0
0,0
50.000
0,0
0,0
0
Kontrol (-) Etil asetat
0
0,0
0,0
0,0
3 Conclusion
Two of secondary metabolites were isolated from seaweed
Halimeda gracillis
namely β-sitosterol from n-hexane
extract and oleic acid from ethyl acetate extract.
The ethyl acetate extract of the active antibacterial is in weak category at a concentration of 200,000 ppm.
Acknowledgments
This research is part of the dissertation. We thank the promoter and co-promoter, Prof. JS. Darmanto, Prof. SB Prayitno, Prof. Ocky
Karna Radjasa for his guidance and assistance. Mr. Prayitno and Ms.Sri Wahyuni as laboratory assistant are also acknowledged.
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