Triticeae Genomics and Genetics 2016, Vol.7, No.02, 1
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soluble carbohydrates remained more or less
unchanged up to 150 mMNaCl, and then it reduced
quickly and highly significantly at 300 mMNaCl
(table 2). In stems the soluble fraction remained more
or less unchanged up to 50 mM and reduced highly
significantly beyond this level, which seemed to be
more or less similar this reduction was about 25%.In
leaves the soluble carbohydrates were reduced
marginally up to 150 mM and highly significantly at
300 mMNaCl. In spikes the soluble carbohydrate in
spike enhanced markedly at the most salinity level
used. The magnitude of this effect was higher at the
moderate doses of the salt.
1.2.2 Soluble protein
In roots the soluble protein of wheat cultivar Sakha 94
enhanced markedly by salinity stress, this
enhancement was more pronounced up to 150
mMNaCl (table 3). In stems, there is a marked
increasing trend in the accumulation of soluble
proteins as the salinity increase in the culture media.
In leaves, the soluble protein content remained more
or less unchanged up to 150 mMNaCl, then some
activation was exhibited which was much more
pronounced and 300 mMNaCl (about 21.7% over the
control). In spike salinity induced an accumulation in
soluble protein reach a maximum value at 150 mM
NaCl levels.
Table 3 Effect of various concentrations of NaCl on protein content as mg / gm dry weight in roots, stems, leaves and spikes of wheat
cultivar cv. Sakha 94 , cv. Gimiza11, cv. Gimiza10 and cv. Giza 168 .
cv. Sakha 94
NaCl Treat.
Root
%
Stem
%
leaf
%
Spike
%
Cont
62.6a
100
50.4a
100
90.9a
100
38.6
100
20 mM
99.3
158.7
58.8
116.6
84.6
93.0
39.9
103.5
50 mM
96.9
154.9
59.6
118.1
72.9
80.1
60.9
157.9
150 mM
99.9
159.7
58.9
116.9
84.0
92.4
50.4
130.7
300 mM
75.6
120.8
84.9
168.4
109.5
120.4
60.2
156.0
L. S. D. 5%
0.21
0.11
0.19
0.13
cv. Gimiza 11
Cont
26.9a
100
32.2a
100
94.2a
100
24.5
100
20 mM
44.6
165.7
36.4
113.0
85.8
91.0
36.4
148.5
50 mM
42.8
159.1
49.4
153.4
83.1
88.2
21.2
86.5
150 mM
53.6
199.2
30.2
93.7
114.9
121.9
21.9
89.3
300 mM
33.0
122.6
29.4
91.3
135.9
144.2
35.6
145.3
L. S. D. 5%
0.17
0.14
0.4
0.10
cv. Gimiza 10
Cont
58.4a
100
101.2a
100
74.8a
100
88.4a
100
20 mM
73.2
125.3
124.0
122.5
80.8
108.0
72.4
81.9
50 mM
61.2
104.7
104.4
103.1
83.0
110.9
75.2
85.0
150 mM
71.2
121.9
104.2
102.9
48.6
64.9
66.4
75.1
300 mM
57.6
98.6
55.855.1
97.2
129.9
63.6
71.9
L. S. D. 5%
0.47
0.23
0.22
0.33
cv. Giza 168
Cont
80.4a
100
93.2a
100
92.4a
100
65.6a
100
20 mM
84.8
105.4
83.4
89.4
88.8
96.1
67.4
102.7
50 mM
86.0
106.9
87.2
93.5
81.6
88.3
65.0
99.0
150 mM
80.4
100
75.2
80.6
77.2
83.5
60.2
91.7
300 mM
87.6
108.9
67.2
72.1
72.0
77.9
52.6
80.1
L. S. D. 5%
0.21
0.51
0.27
0.15
Means not labeled with letter (a) are significantly different from control level mean.
*Significant at < 0.05
In roots of cv. Gimiza 11, the soluble protein content
accumulated hugely and unexpectedly up to 150
mMNaCl approached 2-folds and the lowest
accumulation of protein was reported in plants
