Triticeae Genomics and Genetics 2016, Vol.7, No.02, 1
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16
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accumulation of amino acids in spike of wheat cultivar
Gimiza 10. In roots of cv. Giza 168 amino acids
content reduced slightly by salinity stress which
seemed to be pronounced at the higher doses of the
salt. In stems there is some promotion in the
accumulation of amino acids at the most salinization
levels. However this trend in the accumulation of
amino acids differed greatly from salinization level to
another. In leaves the salinity stress up to 150
mMNaCl induced insignificant changes in the amount
of amino acids, and then there is a marked and
progressive increasing trend in the amount of amino
acids by increasing the salinity stress in the soil. The
magnitude of this promotion was the highest at the
highest salinization level (300 mMNaCl).
At this level the percent of increase in the amino acids
content was 79.1% over the control values. In spikes
the amount of amino acids remained more or less
unchanged up to 50 mMNaCl, and then they reduced
quickly and suddenly by increasing the salinity stress
in the soil. The highest reduction was observed at the
higher doses of salt (300 mMNaCl), which was about
36% below the control values.
Figure 3 Effect of various concentrations of NaCl on proline as mg/ gm dry weight in roots, stems, leaves and spikes of wheat
cultivar cv. Sakha 94, cv. Gimiza11, cv. Gimiza10 and cv. Giza 168.
1.2.4 Proline content
In wheat cultivar Sakha 94, the data in figure 3
showed that the salinity stress dropped greatly the
amount of proline in roots, but more so at the higher
salinity. Proline content is reduced by about 43.5% at
300 mM NaCl. In stems, proline content enhanced
greatly and irregularly at the most salinization levels,
so the amount of proline differed greatly among the
different salinization levels. At the lower salinization
levels while proline content increased by 83% at 50
mMNaCl, it increased by only 8.3% at 20 mMNaCl.
At the highest salinization levels (300 mM NaCl),
the percent of increase in proline at 300 mM NaCl was
50%.Moderate salinization level (150 mMNaCl), the
percent of increase in proline content of stem was
50%.In leaves, proline content remained more or less
Cv. Sakha
Cv. Gimiza 11
0
0.05
0.1
0.15
0.2
0.25
Root Stem Leaf Spike
Cont.
mM 20
mM 50
mM
150
mM
300
Cv. Gimiza 10
Cv. Gimiza 168
