Cancer Genetics and Epigenetics 2016, Vol.4, No.1, 1-10
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re-annotation method (Liao et al., 2011); remove the ambiguity of the probe, to obtain relatively accurate probe
annotation information of coding genes and non-coding genes.
Firstly, we alignment probe sequences with protein-coding genes and non-coding genes use Bowtie (Langmead et
al., 2009), separately. Secondly, we removed the probe both alignment protein-coding gene and non-coding gene.
Finally, we mapped the protein-coding transcripts and non-coding transcripts to gene, and remained the probes
only matched to a gene; removed gene matched less than 3 probes.
Figure 7 Function annotation of ncRNA
A. RP11-700H6.1 function annotation B. CTB-43P18.1 function annotation C. LINC01003 function annotation
D. RP11-49K24.8 function annotation E. RP11-92F20.1 function annotation F. RP11-770J1.5 function annotation