分子植物育种
(
网络版
), 2016
年
,
第
14
卷
,
第
1062-1071
页
Fenzi Zhiwu Yuzhong (Online), 2016, Vol.14, 1062-1071
Copyright © 2016 BioPublisher 1068
向编辑的组成元件转移到植物细胞中最常用的方
法为农杆菌
Ti
质粒介导法，存在浸染效率不高和物
种限制等问题，而一些植物病毒
(
如双生病毒等
(Baltes et al., 2014))
具有浸染效率高和宿主范围广
等优点，将来可能可以改造成为运载这些元件的有
效、简单的工具；
3)
作为“明星”的
CRISPR-Cas9
技术存在
sgRNA
识别位点受限于
PAM
序列的问
题，因此不能识别基因组任意位点，大大限制其应
用，而Ⅲ型
CRISPR-Cas
系统识别位点不受
PAM
的
限制
,
靶位点选择上更自由
(
李君等
, 2013)
。因此
,
今后对
CRISPR/Cas
系统进行更深入的研究和探
索，使该技术具有更广泛的适用性。相信在不久的
将来，随着对人们对生物世界的深入的探索和认
识，高效、特异、简单的基因组靶向编辑技术将会
在植物研究领域大放光彩。
作者贡献
廖鹏飞完成论文初稿的撰写和修改；聂旺、余
雅心和童普国参与参考文献查找和整理以及图表
绘制；李绍波和朱友林指导论文撰写和修改。全体
作者都阅读并同意最终的文本。
致谢
本研究由国家自然科学基金
(31260313)
和江西
省自然科学基金
(20132BAB204012)
共同资助。
参考文献
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