分子植物育种
(
网络版
), 2016
年
,
第
14
卷
,
第
1062-1071
页
Fenzi Zhiwu Yuzhong (Online), 2016, Vol.14, 1062-1071
Copyright © 2016 BioPublisher 1067
表
2 ZFNs
、
TALENs
和
CRISPR-Cas9
在植物中基因组靶向编辑的例子
Table2 Examples of plant targeted genome editing by ZFNs, TALENs and CRISPR-Cas9
靶向编辑技术
Technologies
植物物种
Plant species
被修饰的基因
Targeted gene names
修饰类型
Modification
types
ZFNs
拟南芥
Arabidopsis thaliana
ADH1
，
TT4
(Zhang
et al
., 2010)
NHEJ
烟草
Nicotiana tabacum
SurA
,
SurB
(Townsend
et al
., 2009)
NHEJ
玉米
Zea mays
IPK1
(Shukla
et al
., 2009)
NHEJ; HR
大豆
Glycine max
DCL 1a, DCL1b, DCL4a, DCL4b, RDR6a, RDR6b, HEN1a
(Curtin
et al
., 2011)
NHEJ
TALENs
拟南芥
Arabidopsis thaliana
ADH1
(Cermak
et al
., 2011)
NHEJ
烟草
Nicotiana tabacum
SurA
;
SurB
(Zhang
et al
., 2013)
NHEJ; HR
水稻
Oryza sativa
EBE of AvrXa7and PthXo3 (Li
et al
., 2012);
OsDEP1
,
OsBADH2
,
OsSD1
,
OsCKX2
(Shan
et al
., 2013b)
NHEJ
大豆
Glycine max
FAD2-1A
,
FAD2-1B
(Haun et al., 2014)
NHEJ
小麦
Triticum aestivum
TaMLO
(Wang
et al
., 2014)
NHEJ
短柄草
Brachypodium
BdABA1
,
BdCKX2
,
BdSMC6
,
BdSPL
,
BdSBP
,
BdCOI1
,
BdRHT
,
BdHTA1
(Shan
et al
., 2013b)
NHEJ
西红柿
Solanum
lycopersicum
PRO
(Lor
et al
., 2014)
NHEJ
CRISPR-Cas9
水稻
Oryza sativa
(OsPDS, OsBADH2, Os02g23823, OsMPK2)
(Shan
et al
.,
2013a)
；
(YSA, SPP, ROC5)
(Feng
et al
., 2013)
；
(CAO1, LAZY1)
(Miao
et al
., 2013)
;
(Promoters of
OsSWEET14
and
OsSWEET11
) (Jiang
et al
., 2013)
NHEJ
；
HR
小麦
Triticum aestivum
TaMLO
(Shan
et al
., 2013a)
NHEJ
高梁
Sorghum bicolor
DsRED2
(Jiang
et al
., 2013)
NHEJ
拟南芥
Arabidopsis thaliana
(
AtPDS3, AtFLS2, AtRACK1b, AtRACK1c
) (Li
et al
., 2013);
BR11, JAZ1, GAI
(Feng
et al
., 2013)
NHEJ
本氏烟
Nicotiana
benthamiana
NbPDS3
(Li
et al
., 2013)
;NbPDS
(Nekrasov
et al
., 2013)
NHEJ; HR
西红柿
Solanum
lycopersicum
SlAGO7
(Brooks
et al
., 2014)
NHEJ
甜橙
Citrus sinensis
CsPDS
(Jia andWang, 2014)
NHEJ
地钱
Marchantia
polymorpha
L.
ARF1
(Sugano
et al
., 2014)
NHEJ
