Molecular Plant Breeding 2015, Vol.6, No.21, 1
-
17
9
Continuing table 4
Locus
Marker type
Chromosome Number
of allele
PIC
Fragment
size (bp)
Marker grade for
uniformity assessment
Left primer
（
5'-3'
）
Right primer
（
5'-3'
）
Xwmc720
Genomic-SSR
4D
13
0.81 100-140
3
CACCATGGTTGGCAAGAGA
CTGGTGATACTGCCGTGACA
Xgwm186
Genomic-SSR
5A
7
0.75 132-180
3
GCAGAGCCTGGTTCAAAAAG
CGCCTCTAGCGAGAGCTATG
Xcwem44
EST-SSR
5A/5B/5D
4
0.74 50-70
3
AGTGCACTGCAAACACAGAG
AGCCGTACACCTTCATAGGC
Xwes26
EST-SSR
5B
2
0.38 160-175
3
TCCCGAGAAGAAGCGAGAT
CCCCAGCAGCAGCACCGTCAC
Xgwm617
Genomic-SSR
5A/6A
11
0.76 100-180
3
GATCTTGGCGCTGAGAGAGA
CTCCGATGGATTACTCGCAC
Xwmc1
Genomic-SSR
3B/6A
5
0.47 130-160
3
ACTGGGTGTTTGCTCGTTGA
CAATGCTTAAGCGCTCTGTG
Xgwm459
Genomic-SSR
6A
13
0.82 118-126
3
ATGGAGTGGTCACACTTTGAA
AGCTTCTCTGACCAACTTCTCG
Xbarc196
Genomic-SSR
6D
3
0.42 130-217
3
GGTGGGTTTTATCGAATAGATTTGCT
GCGTTTCGTCAAGATTAATGCAGGTTT
Xcfd33
Genomic-SSR
6D
3
0.51 170-242
3
TACCGCAATAATCACACCCA
GGTCGATGGACTGTCCCTAA
Xgwm469
Genomic-SSR
6D
9
0.8
150-180
3
CAACTCAGTGCTCACACAACG
CGATAACCACTCATCCACACC
Xswes209
EST-SSR
7B
4
0.63 280-300
3
AAGTCCACATTTCAACCCTG
GTTTCACCTCTAGCCTACCC
Xgwm344
Genomic-SSR
7B
8
0.61 130-150
3
CAAGGAAATAGGCGGTAACT
ATTTGAGTCTGAAGTTTGCA
Xgwm44
Genomic-SSR
7D
12
0.84 160-210
3
GTTGAGCTTTTCAGTTCGGC
ACTGGCATCCACTGAGCTG
*: The markers for detection of seed purity
