Molecular Plant Breeding 2015, Vol.6, No.21, 1
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Table 4 Eighty SSR markers used to test seed purity and uniformity of wheat varieties
Locus
Marker type
Chromosome
Number
of allele
PIC
Fragment
size (bp)
Marker grade for
uniformity assessment
Left primer
（
5'-3'
）
Right primer
（
5'-3'
）
Xcwm65*
EST-SSR
1A
10
0.66 220-260
1
TCATTGGTGTCATCCCTCGTGT
GAATAATGCCTTGACCCTGGAC
Xbarc80*
Genomic-SSR
1B
6
0.61 97-125
1
GCGAATTAGCATCTGCATCTGTTTGAG
CGGTCAACCAACTACTGCACAAC
Xcfd72*
Genomic-SSR
1D
11
0.66 210-240
1
CTCCTTGGAATCTCACCGAA
TCCTTGGGAATATGCCTCCT
Xgwm294*
Genomic-SSR
2A
16
0.74
67-105
1
GGATTGGAGTTAAGAGAGAACCG
GCAGAGTGATCAATGCCAGA
Xgwm429*
Genomic-SSR
2B
12
0.67 190-230
1
TTGTACATTAAGTTCCCATTA
TTTAAGGACCTACATGACAC
Xgwm261*
Genomic-SSR
2D
11
0.67 160-210
1
CTCCCTGTACGCCTAAGGC
CTCGCGCTACTAGCCATTG
Xgwm155*
Genomic-SSR
3A/1D
9
0.74 120-160
1
CAATCATTTCCCCCTCCC
AATCATTGGAAATCCATATGCC
Xswes185*
EST-SSR
3B
4
0.58 180-190
1
ACATCATCAACTATGCCGAACA
CGCCTTTAGATCCCACCC
Xgdm72*
Genomic-SSR
3D
10
0.71 120-150
1
TGGTTTTCTCGAGCATTCAA
TGCAACGATGAAGACCAGAA
Xgwm610*
Genomic-SSR
4A
7
0.55 160-175
1
CTGCCTTCTCCATGGTTTGT
AATGGCCAAAGGTTATGAAGG
Xksum62*
EST-SSR
4B
9
0.77 170-210
1
GGAGAGGATAGGCACAGGAC
GAGAGCAGAGGGAGCTATGG
Xbarc91*
Genomic-SSR
2B/4D
12
0.76 110-160
1
TTCCCATAACGCCGATAGTA
GCGTTTAATATTAGCTTCAAGATCAT
Xgwm304*
Genomic-SSR
2A/5A
16
0.80 200-230
1
AGGAAACAGAAATATCGCGG
AGGACTGTGGGGAATGAATG
Xgwm67*
Genomic-SSR
3A/5B/7D
8
0.58
70-100
1
ACCACACAAACAAGGTAAGCG
CAACCCTCTTAATTTTGTTGGG
Xcfd29*
Genomic-SSR
5D
14
0.82 160-190
1
GGTTGTCAGGCAGGATATTTG
TATTGATAGATCAGGGCGCA
Xgwm334*
Genomic-SSR
6A
21
0.80 100-140
1
ATCTGCCTCTTCGCATCATC
CAGTGCTCTTCACTCTCCCC
Xbarc198*
Genomic-SSR
6A /6B
12
0.66 110-160
1
CGCTGAAAAGAAGTGCCGCATTATGA
CGCTGCCTTTTCTGGATTGCTTGTCA
Xcfd76*
Genomic-SSR
6D
14
0.79 140-170
1
GCAATTTCACACGCGACTTA
CGCTCGACAACATGACACTT
Xcfa2028*
Genomic-SSR
7A/7B
7
0.49 230-260
1
TGGGTATGAAAGGCTGAAGG
ATCGCGACTATTCAACGCTT
Xgwm333*
Genomic-SSR
7B
8
0.61 140-150
1
GCCCGGTCATGTAAAACG
TTTCAGTTTGCGTTAAGCTTTG
Xgwm437*
Genomic-SSR
7D
18
0.69
90-140
1
GATCAAGACTTTTGTATCTCTC
GATGTCCAACAGTTAGCTTA
Xbarc17*
Genomic-SSR
1A
8
0.77 250-280
2
GCGCAACATATTCAGCTCAACA
TCCACATCTCGTCCCTCATAGTTTG
Xcwm75
EST-SSR
1A
4
0.50 210-240
2
TGGCAATGCAGCGATGTCT
TTGGGGTGGTTGTTGCTGATA
