Molecular Plant Breeding 2015, Vol.6, No.20, 1
-
10
1
Research Article
Open
Access
Differential expressed genes of banana leaves induced by
Fusarium
wilt disease
Peng T.C., Chen Y.Y., Luo J.Y., Zhao H., Mu L., Xie J., Wei S.S., Xia Y.Q., Tang H.
National Key Laboratory Base of Tropic Biological Resources Sustainable Utilization, College of Agriculture, Hainan University, Haikou 570228, P. R. China
Corresponding
authors
email
Molecular
Plant
Breeding,
2015,
Vol.6,
No.20
doi:
10.5376/mpb.2015.06.0020
Received:
25
Aug.,
2015
Accepted:
26
Sep.,
2015
Published:
27
Oct.,
2015
Copyright
© 2015
Peng et al.,
This
is
an
open
access
article
published
under
the
terms
of
the
Creative
Commons
Attribution
License,
which
permits
unrestricted
use,
distribution,
and
reproduction
in
any
medium,
provided
the
original
work
is
properly
cited.
Preferred
citation
for
this
article:
Peng T.C., Chen Y.Y., Luo J.Y., Zhao H., Mu L., Xie J., Wei S.S., Xia Y.Q. and Tang H., 2015,
Differential expressed genes of banana leaves induced by
Fusarium
wilt disease, Interaction,
Molecular
Plant
Breeding,
6(20):
1
-
10 (doi:
Abstract
Fusarium wilt of banana is a destructive fungus disease caused by
Fusarium oxysporum
f. sp.
Cubense
. This disease had
harmful effects on banana planting and its industrial production. In order to study the pathogenic mechanism of banana Fusarium wilt
disease, we used cDNA-AFLP technique to identify differential expressed transcripts that were induced or inhibited by
Fusarium
oxysporum
f. sp.
Cubense
tropical race 4 infection. Among 223 isolated cDNA fragments, we sequenced and get 137 unique banana
cDNAs that involved in different functions, including disease and defense response, transcription regulation, signal transduction,
primary metabolism, energy metabolism, cell growth and division, protein destination and storage, and so on. Among these genes, 8
ESTs were related to disease and defense response including NBS-LRR type resistance gene, hot shock protein, and alcohol
dehydrogenase; 19 ESTs were related to transcriptional regulation including transcription factor MYB and MYC, zinc finger protein,
and Glycine-rich RNA binding protein; 12 ESTs were related to signal transduction including the Ras-related nuclear proteins. We
choose out 6 important genes to analyze the expression patterns by sqRT-PCR, and confirm their relationship with pathogenic
mechanism of banana Fusarium wilt.
Keywords
Banana;
Fusarium oxysporum
f. sp.
Cubense
; Differential expressed genes; Fusarium wilt disease; Pathogenic response
Introduction
Banana is an important tropical monocot crop species
as food or fruit. Fusarium wilt of banana, known as
Panama disease, is a devastating vascular systemic
disease. In recent years, this disease has burst out in
banana production regions around the world, has
caused great economical loss to farmer, and it is really
a great threat to banana production (De Ascensao et
al., 2000). Fusarium wilt disease is widely distributed
in South Pacific, Asia, Australia, Africa and tropical
America; in China, it mainly exists in Guangdong,
Guangxi, Yunnan, and Hainan provinces (Wei et al.,
2005).
The Fusarium wilt of banana is caused by
Fusarium
oxysporum
f. sp.
cubense
(Foc), a pathogenic soil
borne fungus. This fungus infects banana plants
through the wound of roots and leaves, and it can be
transferred and spread by soil, water and infected
suckers (Van Den Berg et al., 2007).
Fusarium
oxysporum
f. sp.
cubense
has 4 races, the race 4 has
the strongest damage on banana production (Thangavelu
et al., 2012), and the tropical race 4 (TR4) is the most
dangerous fungus in China.
In order to prevent banana Fusarium wilt, the most
economical and fundamental pathway is to breed
disease-resistant varieties, because chemical control
can not reach ideal effects. The discovery of banana
defense mechanism is a prerequisite for disease
control through molecular breeding. In molecular or
transcriptome level, to understand the pathogenic
mechanism of banana to Foc will provide a scientific
basis to develop effective measures to control disease
(Groenewald et al., 2006). Through transcriptomic
profile analysis (Li et al., 2012) found that the
recognition of PAMPs and defense-related genes may
contribute to Foc4 resistance in banana. The sequencing
of banana (AA) genome provides great promotion on
banana research (D’ Hont et al., 2012). The reason for
banana susceptibility to Foc TR4 is that SA
biosynthesis-related genes are suppressed and the
induced resistance of banana against Foc TR4 might
be a case of salicylic acid-dependent systemic acquired
resistance
.
However, the molecular pathological mechanism of
banana to Foc TR4 is not clear. The objective of this
study was to identify some key genes differentially
