Molecular Plant Breeding 2015, Vol.6, No.20, 1
-
10
9
Figure 4 Semi-quantitative RT-PCR showed differential gene
expression of banana leaf between the control and infection
treatments at different stages. Lane 1, 2, 3 and 4 were 4 h, 24 h,
3d, 6d time points in control treatment; lane 5, 6, 7 and 8 were
4 h, 24 h, 3 d, 6 d time points in infection treatment.
of ESTs were got totally, among which 64.96% had
significant homology with known genes, including
12.41% related to primary metabolism, 5.84% related
to energy metabolism, 2.92% related to protein
synthesis, 5.11% related to protein destination and
storage, 13.87% related to transcription, 4.38% related
to transporters, 2.19% related to cell growth and
division, 2.19% related to cell structure, 9.48% related
to signal transduction, 6.57% related to disease and
defense response; the remaining 35.04% ESTs were
unknown about its functions, including 24 new-found
ESTs.
In this research, 8 ESTs related to disease and defense
response were obtained, including NBS-LRR type
resistance gene, hot shock protein, alcohol dehydrogenase,
and so on; 19 ESTs related to transcriptional
regulation, including the transcription factor MYB and
MYC, zinc finger protein, Glycine-rich RNA binding
protein, and so on; 12 ESTs related to signal
transduction, including the ubiquitin-conjugating
enzyme E2 and so on; the remains were related to
primary metabolism, energy metabolism, and so on.
All in all, the pathological response of banana to
Fusarium wilt disease was a complex mechanism,
although the genome of doubled-haploid (2n=22,
1C=523 Mb) from the species
Musa acuminata
(A
genome) subspecies malaccencis had been sequenced
(D'Hont et al., 2012) and provided great promotion for
banana research, more researches are needed to do
deeply to elucidate lots of confusing questions. The
results of this study will provide some clues to reveal
the molecular mechanism of banana infected by
Fusarium oxysporum
f. sp.
Cubense
tropical race 4
.
Acknowledgements
This research was supported by the following grants:
30860149 (National Natural Science Foundation of
China, NSFC), 31360364(National Natural Science
Foundation of China, NSFC), 210172 (Key Scientific
Research Program from the Ministry of Education of
China), lhxm-2012-2 (Joint Support Program from
Tropical Crop Breeding Engineering Center of
Ministry of Education and National Crop Science Key
Discipline of China), and ZDZX2013023 (Key
Scientific Research Program from Hainan Province).
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