Molecular Plant Breeding 2015, Vol.6, No.20, 1
-
10
5
Figure 2 The cDNA-AFLP products of three primer combina-
tions on PAGE gel.
The three primer combinations were Esa10/Msa9, Esa10/
Msa10, and Esa10/Msa11; lane 1, 2, 3 and 4 were cDNA-AFLP
products of 4 h, 24 h, 3d, 6d time points in CK treatment; lane
5, 6, 7 and 8 were cDNA-AFLP products of 4 h, 24 h, 3 d, 6 d
time points in infection treatment
Figure 3 Functional catalogues distribution of ESTs derived
from the cDNA-AFLP
defense response system to react to pathogen. The
receptor on cell membrane will receive and transfer
infection signal into cell, it will activate some genes or
suppress other genes. How banana is responded to
Fusarium oxysporum
f. sp.
Cubense
infection? It is
really an important and complicated question needed
to discover. In this research, 8 ESTs (Table 3) were
classified into the functional catalogue of disease and
defense responses. These genes included in NBS-LRR
type resistance protein, heat shock protein, alcohol
dehydrogenase, and so on.
Table 3 ESTs related to disease and defense response
NO.
GenBank ID of homology Putative function
E62
CCD20161.1
retrotransposon hot spot
protein (RHS)
E113 AAB96976.1
NBS-LRR type
resistance protein
E121 CAA55184.1
heat shock protein
70 kDa
E127 YP_002466452.1
alcohol dehydrogenase
E176 EGY14241.1
chitin synthase
E180 CBA72167.1
low calcium
response protein
E203 ABA97293.1
Zinc knuckle
family protein
E210 XP_002890832.1
zinc ion binding protein
The NBS-LRR type resistance protein has a central
nucleotide-binding site (NBS) domain and a C-terminal
leucine-rich repeat (LRR) domain. LRR has a close
relationship with different proteins interaction and
signal processes, and exists in variety proteins whose
function and cellular localization are different. The
NBS has an N-terminal sub-domain that contains
consensus kinase 1a (P-loop), kinase 2 and kinase 3a
motifs common to a large variety of nucleotide
proteins (Traut et al., 1994). Many plant disease
resistance genes have a great many of NBS resistance
genes which contain a highly conserved amino acid
region, which is closely associated with pathogenic
recognition and defense response. Consequently, the
NBS-LRR genes provide very effective strategies and
methods for exploring, identifying and using the
unknown plant disease resistance gene analogues. Up
to now, many DNA and cDNA sequences that have
homology with the known resistance genes had been
isolated from Arabidopsis, soybean, lettuce, tobacco,
potato, barley, wheat, rice, tomato, apple and citrus
(Yoshimura et al., 1998; Ori et al., 1997; Lee et al.,
2003; Deng et al., 2003). In this study, the banana
NBS-LRR resistant gene (E113) was isolated and its
quantitative expression pattern showed significant
up-regulation after Foc-TR4 infection on leaves,
which indicated the NBS-LRR type resistance gene
may play a certain role in banana
Fusarium
wilt
disease prevention.
Heat shock protein (HSP) is a kind of stress-activated
factor. It is induced by various abiotic and biotic
stresses, such as water stress, salt stress, hypoxia,
