Molecular Plant Breeding 2015, Vol.6, No.20, 1
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ABA, nitrite, cadmium, and virus, and so on. The
diversity of induction factors indicated that plants had
cross-resistance with different stresses and had some
common molecular basis within a variety of adversity
stresses. The heat shock protein had been found from
barley, wheat, millet, soybean, carrot, tomato, cotton,
tobacco, Arabidopsis, and so on. Plants must cope
with various acute environmental changes including
biotic and abiotic stresses. Proteins can be misfolded
and aggregate under stress and lead to many
functional problems in cell, and the misfolded protein
should be refolded to recover the right conformation.
HSPs are molecular chaperones and protein
remodeling factors, some of them are upregulated in
response to stress (Wang et al., 2004). Hsp70 gene in
Trichoderma harzianum conferred tolerance to heat
and other abiotic stresses to this fungus. Transgenic
Arabidopsis thaliana plants expressing the T. harzianum
hsp70 gene exhibited enhanced tolerance to heat stress;
hsp70 confers tolerance to heat and other abiotic
stresses, and the fungal HSP70 protein acts as a
negative regulator to HSF transcriptional activity
in Arabidopsis (Montero-Barrientos et al., 2010). In
this study, the banana hsp70 gene (E121) was isolated
and showed significant down-regulation after Foc-TR4
infection on leaves, hsp70 gene may confer tolerance
to banana
Fusarium
wilt disease.
The alcohol dehydrogenase (ADH) is a metalloenzyme
containing zinc, has a wide range of substrate
specificity, and widely exists in human, animal liver,
plants and microbial cells. ADH plays an important
role in the anaerobic respiration process in plant.
Some studies showed that ADH belonged to the
protein induced by anaerobic, the activity of ADH in
root tissue could greatly increase when terrestrial plant
was under the condition of waterlogged soil or
anaerobic environment. After waterflooding, the
short-term increase of its activity was good for energy
supply of plant (Ning et al., 2009). After plant is
infected by pathogen, the organism will gradually lose
vitality, the metabolic activity also will gradually
weaken and respiration will decrease. By this time, the
activity of ADH will rise, which can supply energy for
plant momently.
The special genes relevant to transcriptional
regulation
Transcriptional regulation is the most important
regulation for plant gene expression. The special
expression of genes is largely regulated on transcriptional
level. When plant is adapted to pathogen infection,
plant obtains a complex defense response mechanism.
After plant identifies pathogen, the related gene
expression of plant defense response is finally induced
through a series of signal transduction (Meng et al.,
2013). In this research, 19 ESTs (Table 4) might be
Table 4 ESTs related to transcriptional regulation
NO.
GenBank ID of homology
Putative function
E01
NP_850477.1
small nuclear ribonucleoprotein D2
E02
ABD32582.1
Integrase, catalytic region; Zinc finger, CCHC-type
E40
XP_002340257.1
C6 transcription factor, putative
E41
YP_004227811.1
LysR family transcriptional regulator
E79
NP_001133511.1
FYN-binding protein
E105
XP_003225943.1
zinc finger protein 420-like
E120
EFN78085.1
Zinc finger and BTB domain-containing protein 17
E134
AAN63154.1
transcription factor MYBS3
E141
ABG65911.1
Zinc finger, C3HC4 type family protein, expressed
E144
ZP_00371933.1
Ribbon-helix-helix protein, copG family domain protein
E157
XP_003614387.1
rRNA intron-encoded homing endonuclease
E159
ABG65969.1
retrotransposon protein, putative, Ty3-gypsy subclass
E185/ E186
AAO46041.1
zinc finger protein ZFP252
E201
AAD15818.1
transcription factor MYC7E
E207
XP_002530460.1
transcription cofactor, putative
E213
XP_002135694.1
GA25504 gene product from transcript GA25504-RA
E216
XP_002768057.1
DEAD box ATP-dependent RNA helicase
E220
XP_003606916.1
Glycine-rich RNA binding protein
