Molecular Plant Breeding 2015, Vol.6, No.16, 1
-
13
3
Table 2 Primer sequences, accession numbers, and characteristics of the 70 SSR markers in 24
Nicotiana
species
Locus
Repeat motif
Primer sequence (5'-3') Forward/Reverse
GenBank Accession no.
No. of alleles
Allele size range (bp)
H
e
H
o
R
PI
TbM1
(ttc)
7
ttgcttagatctggactatccgtag/cggtttttcccttgtatataggtgt
DQ777877
5
150-300
0.32
0.48
-0.12
0.30
TbM2
(tc )
16
agaatactcaagctatgcatccaac/ ttccttagatctggactatgtgctc
DQ865406
6
200-350
0.24
0.49
-0.01
0.18
TbM3
(ac)
14 (n)59 (ac)20 (n)22 (ac)52
agtgattctcttgcttagatctgga/gggaattcgattctcttgcttagat
DQ865407
6
200-700
0.29
0.40
0.19
0.34
TbM4
(ag)
10
ttctcttgcttagatctggactacc/ccctactgttggtttagtaggtgaa
DQ865408
4
150-500
0.17
0.42
-0.09
0.13
TbM5
(tc)
18
tttagtagcacttaggttcctgcat/attcgattctcttgcttagatctgg
DQ865409
5
200-400
0.38
0.43
0.34
0.44
TbM6
(ag)
11
agagtgtacgcagaccttaccctac/atacgactcactatagggcgaattg
DQ865410
5
200-400
0.36
0.46
0.25
0.37
TbM7
(gt)
28
cttgcttagatctggactatgtgct/cacacacacactcagattttctctt
DQ865411
5
190-300
0.11
0.49
-0.27
-0.04
TbM8
(tc)
20
tccctgcatagaactatctcaactc/tcatgctcttcttgtactccctact
DQ865412
6
180-400
0.18
0.49
-0.14
0.08
TbM9
(ct)
19
gtttgaataaccgattgctcgt/ggtgtgctccttggcttagat
DQ865413
6
200-500
0.09
0.41
-0.23
0.01
TbM10
(tc)
22
attgtctgtcttgtacagtctttgg/ccattctcccagaatagctctaagt
DQ865414
7
200-400
0.32
0.47
0.17
0.32
TbM11
(ac)
14
tgattctcttgcttagatctggact/attcgattctcttgcttagatctgg
DQ865415
6
200-300
0.27
0.47
0.07
0.25
TbM12
(tct)
7tg
(ttc)
7
acacgaggcttgattttagcag/tcgaagggttaggagagaagtaatc
DQ865416
5
200-400
0.12
0.46
-0.23
0.01
TbM13
(ga)
29
cagtttaagcatctctagcagggta/caataaacctccttactctctctctcc
DQ865417
6
200-500
0.34
0.50
0.19
0.32
TbM14
(ag )
16
atagcaatcaacaccaacctcat/cttatacttcgtgccatgtcaaagt
DQ865418
7
200-300
0.22
0.48
-0.05
0.16
TbM15
(aga)
7
aaaagcagactctgttctgttgact/ctcttgcttagatctgtgctctctt
DQ865419
6
200-400
0.18
0.20
0.16
0.29
TbM16
(tct)
7
tacttctgctctatacttggggaag/gtaaacaacaggcagcctacaaat
DQ865420
5
200-300
0.30
0.48
0.12
0.28
TbM17
(ac)
14
tgattctcttgcttagatctggact/atacgactcactatagggcgaattg
DQ865421
6
200-500
0.14
0.49
-0.20
0.02
TbM18
(tct)
9
gaatatgtccatggttttctcactc/tgcttagatctggactagaagaagg
DQ865422
7
200-400
0.17
0.43
-0.09
0.13
TbM19
(ct)
11
attcgattctcttgcttagatctgg/tggaattagggattgagagatagag
DQ865423
6
150-500
0.00
0.00
0.00
0.00
TbM20
(ga)
11
gggagcagtttacagtgttgtttat/aaagtgatctcactcctagccatct
DQ865424
6
200-300
0.15
0.15
0.15
0.25
TbM21
(aag)
18
aagagaacagcttgtaaacccaag/agtgattctcttgcttagatctgga
DQ865425
6
150-500
0.16
0.38
-0.07
0.15
TbM22
(atc)
5
(aga)
7
actaggaagtccactctcatttcag / caaagtcagagtgagagaagtgatg
DQ865426
7
200-500
0.18
0.48
-0.12
0.10
TbM23
(tct)
11
ctcggaaaaggtcagaagagaaag/tgattctcttgcttagatctggact
DQ865427
7
200-300
0.32
0.49
0.16
0.30
TbM24
(ag)
10
ctaatttcttactcgaccccatgt/taggtgcctttcttgacatatcttc
DQ865428
5
200-500
0.12
0.31
-0.07
0.13
TbM25
(ga)
11
atctggactagtagtttgcgtgtct/aagaaatctcactcccagtctcttt
DQ865429
5
200-500
0.09
0.46
-0.28
-0.04
TbM26
(tg)
5
(tg)
9
(tg)
5
(tg)
6
gtaacgactactatagggcgaattg/ctcacacacacacatactcacacac
DQ865430
5
150-500
0.13
0.50
-0.24
-0.01
TbM27
(ct)
10
ctggactacaataaacctcatcacc/ggagtagttcacacagctattctgg
DQ865431
6
200-500
0.21
0.44
-0.03
0.18
TbM28
(ggagaa)
6
agaggaagagtagagatcgggatag/aagagtgttgtcacctgctgtct
DQ865432
5
200-300
0.26
0.49
0.02
0.21
TbM29
(tc)
11
tagaagtggaccacctgtcaagtaa/gcagatgaaaagagtgagagtacag
DQ865433
6
200-400
0.16
0.38
-0.07
0.14
