Molecular Plant Breeding 2015, Vol.6, No.16, 1
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Table 4 List of markers for
Nicotiana
species identity and
types/group identity within cultivated tobacco-
Nicotiana tabacum
Species/types
Markers
Species
N. gluaca
TbM44, TbM46, TbM59
N. paniculata
TbM3, TbM29, TbM41
N. thrisiflora
TbM32, TbM41
N. rustica
TbM60
N. undulate
-
N. trigo
TbM52
N. plub
TbM61
N. sylves
TbM49
N. rependa
TbM33
N. nesophila
-
N. corymbosa
TbM60
N. acuminatae
TbM41
N. clevlandii
TbM35, TbM45, TbM47
N. nudicaulis
TbM16, TbM30, TbM31, TbM33,
TbM47
N. suav
TbM7, TbM31
N. debnyi
-
N. gossei
TbM4, TbM20, TbM33, TbM35
N. maritama
TbM43, TbM47
N. velutiana
TbM20, TbM43
N. occi
TbM31, TbM44, TbM48, TbM57
N. simulans
TbM43, TbM46
N. goodsp
TbM37, TbM44
N. rosulata
TbM21, TbM46, TbM46, TbM52
Types
FCV
TbM1, TbM9, TbM17, TbM25,
TbM33
Chewing
TbM6, TbM14, TbM22, TbM30
Chewing (
rustica
)
and Hookah
TbM8, TbM16, TbM24, TbM32
Burley
TbM2, TbM10, TbM18, TbM26,
TbM34
Cheroot
TbM4, TbM12, TbM20, TbM28
Bidi
TbM7, TbM15, TbM23, TbM31
Cigar wrapper
TbM5, TbM13, TbM21, TbM29
Natu
TbM3, TbM11, TbM19, TbM27,
TbM35
revealed that the
Nicotiana
species studied were
separated into three main clusters (Figure 3). The
cluster-I is formed by the 11 species; all the species
are belongs to the subgenus Rustica and Petunoides.
In this cluster the species
gluaca
and
paniculta
formed
small sub cluster (Ia), similarly,
thrisiflora, rustica
and
undulate
formed sub cluster (Ib). Among the cluster- I,
rependa
and
nesophila
were showed higher similarity
(91%), since both are belong to the section Rependae
and have the similar chromosome number 24. The
second cluster formed with 7 species of
Nicotiana,
in
which all the species are belongs to the subgenus
Petunioides and section Suaveolentes except
N.
tabaccum
which belongs to the subgenus Tabacum.
The third cluster is formed by 6 species, among those
3 species belongs to the section Suaveolentes and rest
are belongs to the different sections of subgenus
Petunoides.
2.5 Transferability of tobacco SSRs to other
Nicotiana
species
Amplification of the 70 SSRs in 23 species of
Nicotiana
revealed an amplification of at least one
allele and the cross transferability of these markers
ranged from 95.7% to 100% (Table 5). All the markers
developed in the present study were transferable to
eight species and 26 markers showed 46 exclusive
alleles. However, no exclusive alleles were identified
for
N. undulata
,
N. nesophila
and
N. debanyi
(Table 4).
In general, about 20
Nicotiana
species could be easily
discriminated using a minimal set of 11 markers
(TbM31, TbM33, TbM41, TbM43, TbM44, TbM46,
TbM47, TbM49, TbM52, TbM60, and TbM61).
3 Discussions
Microsatellite markers are considered as versatile
tools for genetic analysis and breeding applications
Figure 3 Grouping of Nicotiana species using SSR markers
