Molecular Plant Breeding 2015, Vol.6, No.16, 1
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Continuing table 2
Locus
Repeat motif
Primer sequence (5'-3') Forward/Reverse
GenBank Accession no.
No. of alleles
Allele size range (bp)
H
e
H
o
R
PI
TbM59
(gaa)
5
n2(ga)
4
caattaaggcattgaacttgc/ttcacccatctcctctctcc
EF375981
9
200-500
0.24
0.49
-0.02
0.18
TbM60
(gag)
6
(gag)
7
cgggaattcgattctcttgc/ggtgtggcggtctagttcc
EF375982
8
200-300
0.11
0.48
-0.25
-0.02
TbM61
(tc)
9
n(tc)
8
aactcctcacgatcccttcc /tccttttagataagcaacttcacg
EF375983
8
200-300
0.26
0.47
0.05
0.23
TbM62
(gaa)
4
ggttgtcgacggacagtagc/ccctccatcctcctcacc
EF375984
6
150-500
0.36
0.45
0.27
0.38
TbM63
(gaa)
5
n9(aga)
5
ggaagttcctacacccaaacc/cctatcttgttccttttcagattcc
EF375985
7
200-500
0.24
0.50
-0.02
0.17
TbM64
(ttc)
3
n30(ttc)
4
ttaaaggaatctggtcgaacg/cacaagcatgaaagataagaaagg
EF375986
7
200-300
0.15
0.50
-0.20
0.03
TbM65
(ag)
9
atgctccttgttccatttcc/aacgcggactttcctattcc
EF375987
8
150-500
0.07
0.36
-0.23
0.00
TbM66
(ga)
9
n2(ag)
3
N2(ag)
6
cgggaattcgattctcttgc/cactagtttgattagtattggcttgc
EF375988
6
150-500
0.18
0.48
-0.11
0.10
TbM67
(aga)
6
(tga)
3
catggaagatgatgaaaatgg/ccgggtcacattcaactgc
EF375989
6
200-300
0.11
0.50
-0.28
-0.04
TbM68
(ctc)
3
n6(ga)
3
gacctgtgccgcaataacc/ctggactaaggacgctttgg
EF375990
6
200-400
0.18
0.20
0.16
0.29
TbM69
(gaa)
4
n82(agg)
6
ggcgataagaactaagaataaagg/tgaaagtcaccgaaagatgc
EF375991
5
200-500
0.32
0.48
0.16
0.30
TbM70
(ct)
9
n7(tc)
17
tctccttgggcatctgtagc/gtttgagcatccctaacaagg
EF375992
6
200-300
0.18
0.20
0.16
0.29
Note:
H
E
, expected heterozygosity;
H
O
, observed heterozygosity;
r
, frequency of null alleles; PI, probability of identity