International Journal of Aquaculture, 2015, Vol.5, No.27 1
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Supplementary Table 1 Pair-wise inter-population diversity, gene differentiation and estimate of gene flow of six different
populations of
Badis badis
Population Pairs
H
T
H
S
D
ST
F
ST
Nm
TR-1and TR-2
0.2954 ±0.0386
0.1183 ±0.0178
0.1771
0.5996
0.3339
TR-1and TR-3
0.2966 ±0.0372
0.1379 ±0.0188
0.1587
0.5352
0.4343
TR-1and TR-4
0.2822 ±0.0385
0.1280 ±0.0178
0.1542
0.5466
0.4147
TR-1and TR-5
0.3124 ±0.0362
0.1276 ±0.0185
0.1848
0.5917
0.3450
TR-1and TR-6
0.3032 ±0.0390
0.1280 ±0.0198
0.1752
0.5778
0.3653
TR-2 and TR-4
0.1848 ±0.0367
0.1220 ±0.0195
0.0628
0.3402
0.9698
TR-2 and TR-5
0.1962 ±0.0404
0.1216 ±0.0220
0.0746
0.3803
0.8148
TR-2 and TR-3
0.2135 ±0.0395
0.1319 ±0.0219
0.0816
0.3822
0.8081
TR-2 and TR-6
0.2037 ±0.0413
0.1220 ±0.0225
0.0817
0.4012
0.7463
TR-3 and TR-4
0.1988 ±0.0406
0.1415 ±0.0276
0.0573
0.2881
1.2355
TR-3 and TR-5
0.2045 ±0.0367
0.1411 ±.0214
0.0634
0.3098
1.1138
TR-3 and TR-6
0.2030 ±0.0377
0.1416 ±0.0229
0.0614
0.3025
1.1529
TR-4 and TR-5
0.1764 ±0.0387
0.1312 ±0.0261
0.0452
0.2560
1.4534
TR-4 and TR-6
0.1955 ±0.0403
0.1317 ±0.0239
0.0638
0.3263
1.0321
TR-5 and TR-6
0.2003 ±0.0391
0.1313 ±0.0227
0.0690
0.3445
0.9514
All Population
0.2983 ±0.0203
0.1304 ±0.0096
0.1010
0.5629
0.3883
H
T
= Expected heterozygosity in random mating total population; H
S
= Mean expected heterozygosity within random mating
subpopulations; D
ST
= diversity among populations; F
ST
= Degree of gene differentiation among populations in terms of allele
frequencies; Nm = Estimate of gene flow
from 6 (OPA-09, OPA-13, OPB-11 and OPB-18) to 13
(OPA-16). Considering all the primers, the size ranges
of the amplified fragments ranged from 150 to 2200
base pairs (Table 1).
1.2
Within and between- population diversity
and
genetic differentiation
The allelic richness or observed number of alleles
ranged from 1.2915± 0.4556 in TR-2 to 1.3769 ±
0.4858 in TR-3 populations. While Shannon’s
Information index (
H´
or
I
) was highest (0.2205 ±
0.2950) in the TR-3 population (Table 2) and the TR-2
population showed its lowest value (0.1648 ±0.2691)
(Table 2). The highest value of evenness (0.922561)
was found in the TR-1 population and the lowest
value of evenness (0.904002) was found in the TR-6
population (Table 2).
The expected (
H
T
) and mean heterozygosity (
H
S
);
diversity among populations (
D
ST
); degree of gene
differentiation (
F
ST
); and estimates of gene flow (
Nm
)
were estimated in pairwise populations. The expected
heterozygosity and mean heterozygosity ranged from
0.1764 ±0.0387 to 0.3124 ±0.0362 and from 0.1183
± 0.0178 to 0.1416 ± 0.0229 respectively
(Supplementary Table 1). The highest observed value
of inter-population diversity (0.1848) was between the
populations of TR-1 and TR-5 and the lowest value
(0.0452) was observed between the populations of
TR-4 and TR-5 (Supplementary Table 1). The degree
of gene differentiation was highest (0.5996) between
the populations of TR-1 and TR-2 whereas the degree
of gene differentiation between the populations of
TR-4 and TR-5 was lowest (0.2560) (Supplementary
Table 1 and Figure. 2). Moreover the populations of
TR-4 and TR-5 showed maximum level of gene flow
(1.4534) between them while the extent of gene flow
was minimum between the populations of TR-1 and
TR-2 (0.3339) (Supplementary Table 1 and Figure. 2).
When total populations were considered the total
genetic diversity (
H
T
) was 0.2983 ± 0.0203 and the
total genetic differentiation coefficient (
F
ST
) among
the populations was 0.5629 (Supplementary Table 1).
1.3
Genetic distance and relatedness
The Nei’s genetic distance between the populations of
TR-1 and TR-5 was maximum (0.5350) and the
distance between the populations of TR-4 and TR-5
was minimum (0.0928) (Figure 3A). The genetic
identity was highest (0.9114) between the TR-4 and
TR-5 populations but the identity between the TR-1
and TR- 5 populations was lowest (0.5857) (Figure
3A). Thus it was observed from the Nei’s genetic distance
