International Journal of Aquaculture, 2015, Vol.5, No.27 1
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10
2
decomposed into two functional components viz.;
species richness (
E
) and evenness (
S
). These three
components add up to
SHE
analysis that may enable
the delineation of change in the diversity pattern
(Buzas and Hayek, 1996). This method allows
researchers to examine the evenness component
separately from the richness and vice-versa in a single
step process (Buzas and Hayek, 1998).
To answer the questions regarding the genetic
variation in any subdivided population or in the
metapopulation, it is very essential to focus our
attention towards the “evolutionary functional unit”
and a population seems the most reasonable level at
which genetic conservation intervention should take
place. The population is where the local adaptation
and genetic changes occur over generations, therefore
allopatric/sympatric population is of great interest. In
a noteworthy book on conservation biology, Meffe
and Carroll in 1997
pointed out one approach to
define ‘evolutionary functional unit’ in a genetic
perspective through a hierarchical genetic analysis of
subdivided populations.
We have previously investigated the present status of
the available genetic diversity in the
Badis badis
populations in the Terai region of West Bengal, India
(Mukhopadhyay and Bhattacharjee, 2014a). We have
also reported the genetic diversity within and between
the populations of
Badis badis
and also determined
the total available genetic diversity present in the
Mahananda-Balason river system of the Terai region
of sub-Himalayan West Bengal, India. Based on this
firsthand information we have carried out further
analyses to extract as much as possible genetic
information regarding this threatened ichthyofauna.
The objectives of the present study was to (1)
ascertain the genetic distance and genetic relatedness
of the different populations of
Badis badis
from the
major river streams of the Terai region of
sub-Himalayan West Bengal, India, (2) determine the
changes in the diversity pattern through
SHE
analysis
among different populations of
Badis badis
from the
streams of the region, and (3) ascertain hierarchical
genetic structure among different
Badis
populations.
1 Results
1.1
RAPD Profile
Twenty-two RAPD primers generated in total 199
amplified fragments from thirty individuals,
corresponding to 6 separate riverine collection sites
(Table 1). The number of amplified fragments ranged
Table 1 Number and size range of fragments amplified by different RAPD primers
Sl/No. Primer
Sequence (5´3´)
G + C Content (%)
Total no. of fragments scored
Size range of fragments (bp)
1
OPA-01
CAGGCCCTTC
70
11
200-1700
2
OPA-02
TGCCGAGCTG
70
11
200-1500
3
OPA-04
AATCGGGCTG
60
8
300-1000
4
OPA-07
GAAACGGGTG
60
12
150-2000
5
OPA-09
GGGTAACGCC
70
6
500-1300
6
OPA-10
GTGATCGCAG
60
7
250-1200
7
OPA-13
CAGCACCCAC
70
6
400-1200
8
OPA-16
AGCCAGCGAA
60
13
500-2200
9
OPA-19
CAAACGTCGG
60
9
400-1600
10
OPA-20
GTTGCGATCC
60
10
400-1800
11
OPB-01
GTTTCGCTCC
60
10
300-1400
12
OPB-03
CATCCCCCTG
70
8
600-1400
13
OPB-04
GGACTGGAGT
60
9
250-1600
14
OPB-05
TGCGCCCTTC
70
8
500-1600
15
OPB-06
TGCTCTGCCC
70
11
250-1500
16
OPB-07
GGTGACGCAG
70
9
500-1500
17
OPB-11
GTAGACCCGT
60
6
300-1700
18
OPB-12
CCTTGACGCA
60
11
150-1800
19
OPB-13
TTCCCCCGCT
70
7
400-2000
20
OPB-15
GGAGGGTGTT
60
12
200-1900
21
OPB-17
AGGGAACGAG 60
9
500-1700
22
OPB-18
CCACAGCAGT
60
6
250-1200
Total = 199
150 – 2200
