International Journal of Aquaculture, 2015, Vol.5, No.27 1
-
10
9
analyze the inter-population genetic differentiation
between six different populations of
Badis badis
,
pair-wise
F
ST
values were calculated using the formula
F
ST
= 1- H
S
/H
T
(where
H
S
is the average expected
heterozygosity estimated from each subpopulation and
H
T
is the total gene diversity or expected heterozygosity
in the total population as estimated from the pooled
allele frequencies) (Wright, 1969). The
F
ST
is
equivalent to the coefficient of gene differentiation
G
ST
(
G
ST
=D
ST
/H
T
). The diversity among populations
(
D
ST
= H
T
- H
S
) was also calculated to ascertain the
inter-population diversity between different pair-wise
populations. The estimated gene flow (
Nm
) between
the pair-wise populations was also calculated by the
formula
Nm=0.5(1-G
ST
)/G
ST
(McDonald and McDermott,
1993). The binary matrix prepared from all scored
fragments were used to generate Nei's unbiased
measures of genetic identity and genetic distance
matrix (Nei, 1978) using the software Popgene ver.
1.32 and the output data matrix was also verified
separately using the software TFPGA ver. 1.3 and
Arlequin ver. 3.1 (Excoffier and Schneider, 2005).
The Nei’s genetic distance matrix was subjected to
unweighted pair-group method using arithmetical
averages (UPGMA) to generate a dendrogram through
linkage procedure using the software Phylip ver. 3.69
(Felsenstein, 2005) and FigTree ver.1.3.1 (Rambaut,
2010). The Nei’s genetic distance matrix was then
used as the basis for ordination by Principal Co-ordinate
Analysis (PCoA), which was performed to show the
distribution of the genotypes in a scatter plot using
GenAlEx ver. 6.5.
Author’s Contribution
TM performed literature search, data acquisition, experimental
studies, data analyses, and manuscript preparation. SB designed
study, defined intellectual content and performed data analyses,
manuscript editing and review.
Acknowledgments
The work was supported by a research grant from University
Grants Commission (UGC), India [MRP Sanction No.:
40-289/2011 (SR)] awarded to corresponding author.
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