Molecular Plant Breeding 2020, Vol.11, No.24, 1-8
1
Research Article Open Access
Transciptome and Metabolomics Analysis of Pepper (Yin Chuan Cavel) Male
Sterility Lind
Xiujuan Yan
1
Xin He
2
Yunxia Zhao
1
Jingxia Gao
1
Xuemei Wang
1
1 Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan Ningxia, 750002, China
2 General Station of Forest Pest Management and Quarantine of Ningxia, Yinchuan Ningxia, 750001, China
Corresponding author Email:
Molecular Plant Breeding, 2020, Vol.11, No.24 doi:
10.5376/mpb.2020.11.0024
Received: 25 Sep., 2020
Accepted: 27 Sep., 2020
Published: 20 Oct., 2020
Copyright © 2020
Yan et al., This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the
paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium,
provided the original work is properly cited.
Preferred citation for this article
:
Yan X.J., He X., Zhao Y.X., Gao J.X., and Wang X.M., 2020, Transciptome and metabolomics analysis of pepper (Yin Chuan Cavel) male sterility lind,
Molecular Plant Breeding, 11(24): 1-8 (doi:
10.5376/mpb.2020.11.0024
)
Abstract
In order to study the relation of different gene expression and Metabolomics difference of pepper (Yin Chuan Cavel) male
sterility lind pollen abortion. The transciptiome was sequenced using RNA-seq technology, the metabolomics was tested by
UPLC-MS/Ms. The result was analyzed by GO categories and KEGG enrichment analysis. A total of 3 319 different expression genes
were successfully obained, include up genes 800, down genes 2 519. Of the 536 metabolites identified, a total of 102 metabolites were
significantly differentiated. The differential metabolites involved up regulation metabolites 68, regulation metabolites 34. Included 17
amino acid derivatives, 16 Nucleotides and derivatives, 13 glyceryl ester, 10 sphingolipids, 8 flavonoids and phenolic acids, 6 in other
classes (mainly related to carbohydrate metabolism). Differential metabolites and significant differential expression genes are mainly
concentrated in protein related amino acid metabolism pathway and flavonoid metabolism pathway. The results showed that the
significant differential expression of transcription genes, through the regulation of metabolism related enzymes, resulted in the
differential metabolism of lipid, Phenolic acids and carbohydrate metabolites, resulted in pollen abortion of the Pepper (Yin Chuan Cavel)
male sterile.
Keywords
Pepper; Male sterility; Transciptome; Metabolomics
Pepper is a very important vegetable crop and loved by the Chinese. It is nutritious and can stimulate appetite,
reduce blood pressure, improve human resistance (Cai et al., 2010). China is a big country of pepper cultivation,
production and processing. In recent years, the planting area of pepper is increasing, and the output ranks first in
the world. With the development of industry, the requirements of pepper breeding and seed production are also
increasing (Suo, 2011). Seed production with male sterile materials is an effective method to simplify seed
production procedures, reduce seed production costs, and ensure seed purity and quality. The correlation between
transcriptome and metabolome of Pepper (Yin Chuan Cavel) Male Sterility Lind was studied, which has important
theoretical significance for further understanding the pollen abortion mechanism of its male sterile line (Chen et
al., 2011).
RNA-Seq method is widely used in plant differential gene expression analysis and new gene mining research, can
effectively provide more information on biological transcription level (Trapnell et al., 2010; Liao et al., 2017; Qin
et al., 2020). Transcriptome and metabonomics integration analysis refer to the normalization and statistical
analysis of the batch data of different biomolecular levels from transcriptome and metabolomics, so as to establish
the data relationship among different levels of molecules (Ding et al., 2019). Combined with functional analysis,
metabolic pathway enrichment, correlation analysis and other biological function analysis, this experiment
systematically and comprehensively analyzed the biological molecular function and regulatory mechanism of
differentially expressed transcriptome in the anther of Pepper (Yin Chuan Cavel) Male Sterility Lind. Screened
out the key metabolic pathways, differentially expressed genes and differentially accumulated metabolites (Chen
et al., 2013). To further understand the mechanism of pollen abortion caused by differential gene expression
regulating substance metabolism.
