Molecular Plant Breeding 2020, Vol.11, No.24, 1-8
5
Figure 5 Analysis of pvalue histogram by enrichment of differential gene and differential metabolite KEGG
1.6 Analysis of O2PLS
All the differential genes and metabolites were selected to establish O2PLS model, and the important variables
influencing the other omics were screened out. Due to the large number of genes and metabolites, only the top 10
genes and the top 10 metabolites that have great influence on the other omics are listed. The results are shown in
Table 1. The top ten metabolites of differentially expressed genes were mainly glycosides, amino acids and
phenolic acids. The results showed that the expression regulation of differential genes between male sterile lines
and fertile lines resulted in significant differences in transcriptome, which resulted in significant regulation of
material metabolism, especially glycoside energy metabolism related substances.
Table 1 Significant difference genes and differential metabolites
Metabolites ID
Chemical material
Gene ID
Length (bp)
mws0913
Trifolin
Capana10g000707
0.068908
pme0088
Luteolin
Capana01g003618
0.06124
mws4176
DL-Alanyl-DL-phenylalanine
Capana03g004625
0.058896
mws0216
Trans-4-Hydroxy-L-proline
Capana02g002479
0.056248
pme0274
6-Aminocaproic acid
Capana00g003963
0.051817
pme2433
Diethanolamine
Capana03g000102
0.051389
mws5042
Glycylphenylalanine
Capana03g004566
0.051224
mws0064
Eriodictyol
Capana12g000531
0.051129
mws0671
L-Homoserine
Capana09g002327
0.050909
mws0636
Phe-Phe
Capana04g000466
0.049434