International Journal of Horticulture, 2015, Vol.5, No.21, 1-45
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the authors.
Budwood of different rose cultivars were treated with 2, 3 and 4 Krad of gamma rays and eyes were budded on
Rosa indica
var. Odorata root stock. Mutations in flower color were detected in some treated plants in the form of
chimera. The chimeric mutants have been established in pure form and all the mutants are being maintained at
Floriculture Laboratory, National Botanical Research Institute, Lucknow, India. For the present RAPD analysis a
number of somatic flower color mutants and their respective parents were selected. The details of the mutant and
parent varieties have been shown in Table 5 and Figure 2.
Table 5 Original and mutant rose cultivars included in RAPD analysis
Name of Variety
Type
Colour
Dose
Year
Parent(P)/Mutant(M)
[gamma rays]
‘Contempo’ [P]
Floribunda
Copper orange
With yellow eye
‘Contempo New’ [M]
Floribunda
Pinkish red
3krad
_
‘Contempo Pink’ [M]
Floribunda
Pink
3krad
1986
‘Contempo Stripe’ [M]
Floribunda
Light yellow
4krad
1983
Stripe on orange
background
‘Contempo Tangerine’ [M]
Floribunda
Tangerine orange
3krad
1984
‘Contempo Yellow’ [M]
Floribunda
Empire Yellow
3krad
1984
‘Imperator’ [P]
Floribunda
Cherry Red
3krad
‘Imperator Stripe’ [M]
light pink stripe
3krad
1986
[Twinkle]
On cherry red
background
‘Imperator Pink’[M]
Pink
3krad
1986
‘First Prize’ [P]
Hybrid Tea
Blend of light
Red & deep pink
‘First Prize
Hybrid Tea
Light pink
3krad
1989
Lighter’ [M]
‘America’s Junior
Floribunda
Coral pink
Miss’ [P]
‘Sukumari’[M]
Floribunda
light pink
3krad
1984
‘Sylvia’[P]
Hybrid Tea
pink
‘Sylvia White’
Hybrid Tea
White
3krad
2002
‘Mrinalini’[P]
Hybrid Tea
Phlox pink
‘Mrinalini Lighter’[M]
Hybrid Tea
Light pink
4krad
1989
‘Mrinalini Stripe’ [M]
Hybrid Tea
White stripe on
3krad
1991
Pink background
1.2 Methods
1.2.1 Cytology
Study of mitosis was done preparing slides by squash technique. First of all fresh roots were pretreated in para
dichlorobenzene and a pinch of Aesculine for 5 min in -20
℃
followed by 15 min at 4
℃
and then fixed in the
fixative propionic acid: alcohol (1:3). Roots were hydrolysed in 1(N) HCl for 13 min and stained by usual Feulgen
staining procedure (Datta, 1976; 1997).
1.2.2 Micromorphology
Flower petals were fixed and then mounted on SEM stubs using double sided adhesive tapes after critical point
drying. Subsequently the materials were sputter coated with gold (200 A
o
thickness) and scanning
pohotomicrographs were taken in JEOL-JSM 35C Scanning Electron Microscope at 10 kV (Datta and Shome,
1994).
.
