International Journal of Horticulture, 2015, Vol.5, No.21, 1-45
8
Figure 1 Amplification profiles of 24
Hippeastrum
cultivars with P3 (A) and P11 (B) primer
Note: M, 0.5 kbp DNA ladder. Lane 1-24 (see SL. Number from Table 1)
Bougainvillea
(Fam. Nyctaginaceae) is one of the most important perennial ornamental shrubs, sometimes
climbers, in tropical and subtropical gardens. NBRI, Lucknow is maintaining a rich
Bougainvillea
germplasm
collection of approx. 180 species/cultivars comprising both single bracted and double or multi-bracted varieties.
NBRI has detected, induced, isolated, established and commercialized a series of new varieties developed through
spontaneous mutation, selective hybridization, chromosome management through colchicine treatment and
gamma ray-induced mutation. The existence of a very large number of cultivars and clones, maintained by
vegetative propagation, reinforces the need of a reliable verification of cultivar identity for nurserymen and
growers. In this study we used several primers to investigate the potential and limits of the RAPD technique for
discriminating among several cultivars and species of
Bougainvillea
and revealing the relationships between them,
regardless of their ancestral lineage, since most of the commercial cultivars are very old and their pedigree is
unknown. List of the germplasms of different groups of bougainvillea used for characterization are given in Table
2.
Table 2
Bougainvillea
cultivars included in RAPD analysis
No.
Proposed
phenotypic group
Name of the cultivars
Origin
Morphology of bract (colour and shape)
1
Buttiana
‘Mrs. Butt’
Basic species of Buttiana 2.59cm X 1.81 cm, crimson
2
,,
‘Mahara’
Bud sport of ‘Mrs Butt’
2.51 X 1.49cm, mutibracted, Rhodamine purple
3
,,
‘Mrs. Mc Clean’
Bud sport of ‘Mrs Butt’
Bracts 2.9 cm X 2.3 cm, Empire Rose. Young
bracts apricot orange changing to pale mauve
4
,,
‘Scarlet Queen’
Bud sport of ‘Mrs Butt’
2.59cm X 1.81 cm, crimson
5
,,
‘Blondie’
Seedling of ‘Mrs. Butt’ Bracts 3.9cm X 2.6 cm, Saturn red later changing to
Amaranth rose, elliptic with acute tip.
6
,,
‘Brilliant’
Seedling of ‘Mrs. Butt’ Bracts 4.7 cmX3.9 cm, brilliant flame colour,
ovate and nonpersistance when died.
7
,,
‘Golden Glow’
Seedling of ‘Mrs. Butt’ Bracts small 4.0cmX 3.5 cm, young bracs ar
majolica yellow, later turning to cadmium orange
finally to light carmin
8
,,
‘Poultoni Special’
Seedling of ‘Mrs. Butt’ Bracts bery big 6.0 cm X 5.5 cm infact one of the
biggest bacted, Solferino Purple, twisted and
clasping each other
9
,,
‘Alick Lancaster’
Bud sport of
B. X buttiana
var. ‘Scarlet Queen’
Bracts 3.5 cmX 2.5 cm,Imperfect, cyclamen purple,
young bracts pale liliac, later turning to purple,
elliptical, with acue tip and cordate base
10
,,
‘Scarlet
Queen
Variegata’
Bud sport of
B. X buttiana
var. ‘Scarlet Queen’
2.61cm X 1.79 cm, Bracts deep carmine.
11
,,
‘Nirmal’
Bud sport of ‘Mrs.
McClean’
Bracts 2.81 cm X 2.22 cm, Bract deep rose in
colour
