International Journal of Horticulture, 2017, Vol.7, No.3, 20-25
24
need of the world. These observations agree with the earlier findings (Gorney et al., 2006; Hu et al., 2006;
Mohibullah et al., 2012). Significant genotypic differences were observed for all traits, which are in agreement
with the results observed by (Stolt et al., 2006). While, negative correlation in spike length was found with
1000-grain weight, which are at par with the results obtained by (Mohibullah et al., 2013). The parameters
showing significant positive correlation can be utilized for further crop improvement program to develop new
wheat varieties.
Allelic variation through SDS-PAGE techniques for HMW glutenin sub-units the quality score obtained in these
landraces are quite comparable to the scores obtained by earlier researchers (Bhagwat and Bahatia, 1988; Lukow
et al., 1989). Glutenins and gliadins are two major groups of the seed storage proteins in hexaploid wheat. The
inheritance of protein is well characterized and their high levels of polymorphism, has proved immense value in
breeding (Morgunov et al., 1990). The HMW glutenin sub-units encoded by genes at
Glu-A1, Glu-B1 and Glu-D1
loci on chromosomes 1A, 1B and 1D respectively have received special attention. The results showed a broad and
interesting range of variation for HMW glutenin sub-units genes in the landraces under investigation which are in
agreement with several researchers viz. (Tahir et al., 1996; Rodriguez-Quijano et al., 1998; Igrejas et al., 1999),
who have reported the higher frequency of 7+8 allele than 17+18 at this locus. The allelic result demonstrated that
the pattern of HMW glutenin sub-units in the said landraces was almost similar to those reported by different
researchers i.e. (Liu et al., 2007; Ren et al., 2008; Alvina et al., 2012; Naushad et al., 2012).
5 Conclusion
From the observations got through frequency distribution it was decided that genotypes [PARC/MAFF 004775
(01)], 004280 (01) and 004279 (05) has maximum spike length of 21.9, 18.7 and 15.2 (cm), while 20.7, 29.8 and
17.6 spikelets spike
-1
and grain yield plant-1consisting of 3.16, 2.53 and 4.58 (g) respectively. 1000-grain weight
for the said genotypes contains 32.2, 25.5 and 45.8 (g). While, grain yield consists of 4761, 4653 and 5058
(Kg/Ha). The observations and evaluation of experimental findings of one hundred germplasms through
SDS-PAGE techniques, it was concluded that a considerable variation in total 12 different HMW glutenin subunit
compositions was found. The frequency of 7+8 and 2+12 was the highest in the entire set of all germplasms.
During the present investigation fifteen germplasms (PARC/MAFF 4272 (01), 4269 (01), 4358 (01), 4355 (02),
PARC/JICA 3835 (05), PARC/MAFF 4358 (03), 4292 (01), 4354 (02), 4354 (01), 4264 (03), 4280 (03), 4269
(02), 4279 (01), 4277 (01) and 4277 (02) possessing 5+10 allele, which is a known source for good bread making
quality, have been identified. The said information’s could be of immense value for varietal improvements in term
of bread making quality. Hence all the said noted accessions have the required potential and recommended for
general cultivation for further breeding program.
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