International Journal of Horticulture, 2017, Vol.7, No.3, 20-25
20
Research Report Open Access
Allelic Variation and Correlation Analysis in Bread Wheat (
Triticum Aestivum
L.) Accessions Based on Various Polygenic Traits
Mohibullah Muhammad
1
,
Rabbani Malik Ashiq
2
, Amin Adnan
3
, Rehman Habibur
1
, Zakiullah
1
, Irfanullah
1
, Muzammail
3
, Islam
Tahir
1
, Ihteramullah
1
, Khakwani Abdul Aziz
1
, Ghulam Said
1
, Shaheen Salma
1
, Qudratullah
1
, Batool Kiran
1
1 Faculty of Agriculture, Gomal University, KP, Pakistan
2 National Agriculture Research Center (NARC), Islamabad, Pakistan
3 GCBB, Faculty of Agriculture, Gomal University, KP, Pakistan
Corresponding email
International Journal of Horticulture 2017, Vol.7, No.3 doi
Received: 04 Nov., 2016
Accepted: 20 Dec., 2016
Published: 03 Feb., 2017
Copyright
©2017 Mohibullah et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Mohibullah M., Rabbani M.A., Amin A., Rehman H., Zakiullah, Irfanullah, Muzammail., Islam T., Ihteramullah, Khakwani A.A., Ghulam S., Shaheen S.,
Qudratullah, and Batool K., 2017, Allelic Variation and correlation analysis in bread wheat (
Triticum Aestivum
L.) accessions based on various polygenic traits,
International Journal of Horticulture, 7(3): 20-25 (doi
Abstract
To evaluate frequency distribution, correlation coefficient and allelic diversity of different wheat (
Triticum Aestivum
L.)
germplasms, an experiment was done for one hundred wheat accessions during the rabi season 2004-05 with augmented field design
at experimental area of Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan. Data were taken for different
morphological parameters i.e. spike length (cm), number of spikelets spike
-1
, grain yield plant
-1
, 1000-grain weight and grain yield
(Kg/Ha). The frequency distribution for spike length ranged from 6.2 to 22.1 (cm) with coefficient of variation 18.63%. The variation
for grain yield plant
-1
ranged from 1.26 to 4.58 (g) with coefficient of variation 21.89%. 1000-grain weight (g) varied from 15.74 to
46.65 (g) with coefficient of variation 23.55%. While, frequency distribution for grain yield (Kg/Ha) ranged from 2 610 to 5 065.9
(Kg/Ha). Hence, Spike length revealed significant and highly positively correlation with number of spikelets spike
-1
, grain yields
plant
-1
and grain yield (Kg/Ha). While, highly significant positive correlation was observed of 1000-grain weight with grain yield
(Kg/Ha). In the 2
nd
part of experiment, SDS-PAGE analysis was utilized to study allelic diversity for high molecular weight glutenin
subunit composition in the said 100 germplasm of wheat (
Triticum Aestivum
L.). All the accessions were partitioned into eighteen
different HMW- glutenin subunit combinations, in which the subunit “null” (48%) has maximum frequency at the Glu-A1 locus. The
most frequent HMW glutenin subunits at Glu-B1 locus were 7+8 with 54%. While, at locus Glu-D1 the subunit 2+12 was found in
most of accessions with frequency of 79%. Hence in the experimental study fifteen accessions (PARC/MAFF 4272 (01), 4269 (01),
4358 (01), 4355 (02), PARC/JICA 3835 (05), PARC/MAFF 4358 (03), 4292 (01), 4354 (02), 4354 (01), 4264 (03), 4280 (03), 4269
(02), 4279 (01), 4277 (01), 4277 (02) were investigated for a desired quantitative traits with known sub units for good bread making
quality, which leads to improvement in further wheat breeding programmes.
Keywords
Pakistan; Wheat; Allelic; Glutenin; Accessions
1 Introduction
Wheat is the staple food for 35% of the world’s population and is grown on 17% of the cultivated area in the
world (Kronstad, 1998). In Pakistan it is called Kanak (Punjabi), Ghanum (Pashto), and Gandum (Urdu). Wheat
belongs to the triticeae tribe (Dumort; Hordeae, Benth.) and subtribe Triticinae of the grass family Poaceae
(Gramineae), one of the largest families of Angiosperm (flowering plants) including 600-700 genera and
approximately 10,000 species. According to (Levy and Feldman, 2002), all major types of polyploids
(autopolyploids, allopolyploids and segmental polyploids) are the members of this family. Being of great
importance nutritionally to many people of the world, wheat grain protein plays a fundamental part in food
processing, for instance, in bread making, biscuits, breakfast cereals, and pasta products (Payne and Rhodes,
1982). Vavilov (1926) a geneticist and plant geographer, explored the agricultural flora in many of the less
developed and largely mountainous parts of the world, where the indigenous crop varieties had not yet given way
to cultivars selected by plant breeders. The amount and the quality of gluten, a large complex of polymeric and
monomeric endosperm proteins chiefly determine the quality of wheat flour which plays a fundamental part in
food processing i.e. in bread making, biscuits, breakfast cereals, and pasta products (Payne and Rhodes, 1982). A
