International Journal of Aquaculture, 2017, Vol.7, No.5, 31
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F4. One way analysis of variance (ANOVA) revealed significant (p < 0.05) increases in the serum globulin
concentration of fishes fed diet F1 and F2 compared with fishes fed the control diet (Diet F0) and significant (p <
0.05) decreases in the serum globulin concentration of fishes fed diets F3 and F4 compared to the fish fed the
control diet (Diet F0). Furthermore, a similar trend was observed 28 days post commencement of the feeding
experiment, although at this time point, there was mild hyperglobulinemia in fishes fed diet F2 compared with fish
fed the control diet. ANOVA revealed this value was not significantly different (p > 0.05) from the values
obtained for the fish fed the control diet.
Table 2 Ingredients, Proximate and Fumonisin B1 Analysis of the Experimental Diets
Fumonisin B1 Amended Experimental Diets
Ingredients
Diets
F0 (0.0 mg
FB
1
/kg)
F1
(10.0
mg
FB
1
/kg)
F2
(20.0
mg
FB
1
/kg)
F3
(40.0
mg
FB
1
/kg)
F4 (80.0 mg
FB
1
/kg)
Fish meal (g)
19.00
19.00
19.00
19.00
19.00
SoyBean cake (g)
37.00
37.00
37.00
37.00
37.00
Maize (g)
32.25
32.23
32.23
32.25
32.25
Palm oil (g)
1.00
1.00
1.00
1.00
1.00
Fish oil (g)
6.00
6.00
6.00
6.00
6.00
Vit/min Premix (g)
0.50
0.50
0.50
0.50
0.50
Bone meal (g)
1.00
1.00
1.00
1.00
1.00
Salt (NaCl) (g)
0.23
0.25
0.25
0.25
0.25
Fumonisin B1 (mg)
2.37
14.68
24.74
43.04
82.77
Starch Binder (g)
2.00
2.00
2.00
2.00
2.00
Crude Protein (%)
40.01
40.00
40.04
40.01
40.02
Gross Energy
19.77
20.00
20.01
19.86
20.00
Digestible Energy (kj.g
-1
) 12.00
12.01
11.98
12.01
12.10
Table 3 Serum biochemistry findings at 7 days of feeding FB
1
based diets
Analyte
Experimental Diets
F0
F1
F2
F3
F4
TP (g/dL)
3.78±1.22
a
3.65±1.00
a
4.04±0.11
a
2.51±1.10
b
2.29±1.01
b
ALB (g/dL)
1.28±1.09
a
1.38±0.96
a
0.87±0.17
a
1.34±0.98
ab
1.95±1.03
b
GLO (g/dL)
2.50±0.13
a
2.27±0.04
b
3.17±0.45
c
1.17±0.13
d
0.34±1.11
e
UREA (g/dL)
2.50±0.01
a
2.72±0.03
b
2.70±0.01
b
2.75±0.01
b
2.81±0.11
c
CREAT (IU)
0.17±0.01
a
0.23±0.03
b
0.22±0.11
b
0.40±0.10
c
0.41±0.01
c
AST (IU)
76.19±1.28
a
99.37±2.30
b
78.39±2.09
a
120.62±6.55
c
131.02±7.95
d
ALT(IU)
17.94±2.01
a
57.09±1.41
b
38.11±2.17
c
30.99±3.01
d
40.13±1.22
e
ALP(IU)
6.17±1.00
a
7.34±1.03
b
8.11±1.35
b
21.30±1.94
c
23.00±1.66
d
LDH(IU)
198.07±6.44
a
600.21±16.59
b
698.10±8.22
c
760.46±9.13
d
739.89±10.28
e
Note: Rows with different superscripts are significantly different from one another. TP= Total protein; ALB= Albumin; GLO=
Globulin; UREA= Urea; CREAT= Creatinine; AST= Aspartate transaminase; ALT= Alanine transaminase; ALP= Alkaline
phosphatase; LDH= Lactate dehydrogenase
Dietary fumonisin B1 elicited marginal but significantly increased (p < 0.05) serum creatinine kinase activity.
ANOVA revealed the serum creatinine kinase activity of fishes fed diet F1 were not significantly (p > 0.05)
different from those of fishes fed diet F2 at 7 days post commencement of the feeding. Also, at the same time
interval, the serum creatinine kinase activity of fish fed diet F3 were not significantly different (p > 0.05) from
those of fish fed diet F4. At day 28 and 56 of the feeding exposure, whereas there were no variations (p > 0.05) in
the serum creatinine kinase activity of fish fed diet F1 and F2 compared to the fish fed the control diet (diet F0);
there were significant variations (p < 0.05) in the serum creatinine kinase activity in fish fed diets F3 and F4
