International Journal of Aquaculture, 2016, Vol.6, No.4, 1
-
9
1
Research Article
Open Access
Locus Specific Genetic Diversity and Population Differentiation Analysis of
Badis badis
(Hamilton-Buchanan, 1822) in Sub-Himalayan West Bengal, India.
Mukhopadhyay T., Bhattacharjee S.
Cell and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, P.O. North Bengal University, Raja Rammohunpur, Siliguri,
District Darjeeling, West Bengal, PIN-734 013, India
Corresponding author Email:
International Journal of Aquaculture, 2016, Vol. 6, No.4 doi:
Received: 22 Feb., 2016
Accepted: 12 Apr., 2016
Published: 26 Apr., 2016
Copyright © 2016
Mukhopadhyay and Bhattacharjee , This is an open access article published under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Mukhopadhyay T. and Bhattacharjee S., 2016, Locus Specific Genetic Diversity and Population Differentiation Analysis of
Badis badis
(Hamilton-Buchanan,
1822) in Sub-Himalayan West Bengal, India., International Journal of Aquaculture, 6(4): 1-9 (doi:
)
Abstract
Badis badis
is a threatened ornamental freshwater fish in the Indian scenario. We have studies RAPD based locus specific
population genetic structure of this species in the eastern sub-Himalayan hotspot region of West Bengal state of India, known as the
Terai and Dooars. We have studied six populations of
Badis badis
from the Terai and seven populations from Dooars region riverine
streams. We have used twenty different RAPD decamer primers which allowed amplification of 184 loci in the Terai and 134 loci in
the Dooars region
Badis
populations. Locus specific observed number of alleles, effective number of alleles, Shannon’s diversity
index and Nei’s genetic diversity were analyzed. The population differentiation of different
Badis badis
populations was measured
through PhiPT analyses.
Keywords
Badis badis
; RAPD fingerprinting; Locus specific diversity; Population differentiation
1 Introduction
Dwarf Chameleon fish
Badis badis
(Hamilton-Buchanan 1822) (Family Badidae) is a freshwater fish species
found in the hill streams of the eastern sub-Himalayan region of West Bengal, India, known as the Terai and
Dooars. It has an important ornamental and commercial value and has been reported as a Vulnerable (VU)
category by National Bureau of Fish Genetic Resources (NBFGR), India (Lakra et al., 2010). However, the
molecular characterization with regard to the genetic background of this fish species has not been carried out in
this eastern sub-Himalayan hotspot region. Therefore, it is essential to ascertain the genetic architecture of any
threatened species having economic value for its management, conservation and most importantly proper
reestablishment in natural population to prevent its extinction from the wild. Since scanty of genomic data is
available in
Badis badis
species till date, we have used RAPD-PCR fingerprinting (Welsh and McClelland, 1990;
Williams et al., 1990), an efficient and inexpensive molecular tool, to investigate and characterize the genetic
background of
Badis
sp through the study of DNA polymorphisms. We have studied the locus specific genetic
diversity and population differentiation between different populations of
Badis badis
from terai and dooars region
of West Bengal, India. This locus specific diversity analysis will help to ascertain further calculation of diversity
indices of this fish species in the study region.
2 Results
The present study revealed that a significant number of loci were amplified using twenty decamer arbitrary RAPD
primers. Six populations (consisting of thirty individuals) of
Badis badis
from the Terai region generated total 184
numbers of amplified fragments correspond to 184 numbers of amplified loci (Table 1). Whereas, seven
populations (consisting of seventy individuals) of
Badis badis
from the Dooars region generated total 134
numbers of amplified fragments correspond to 134 numbers of amplified loci (Table 2). The observed number of
alleles, effective number of alleles, Nei’s genetic diversity and Shannon’s diversity index in Terai region
populations were 1.9598±0.1969, 1.4846±0.3008, 0.2983± 0.1425 and 0.4589±0.1834 respectively (Table 1). The
