巩鹏涛等：蛋白质基因组学研究中的质谱仪与生物信息学方法
Copyright © 2015 BioPublisher Jisuan Fenzi Shengwuxue | Vol.4 | No.1 | 1–12
表
1
可用于蛋白质分析的质谱仪
(Thelen and Miernyk, 2012)
Table 1 Overview of mass spectrometers available for protein analysis (Thelen and Miernyk, 2012)
型号
厂商
分析仪
精度
(PPM)
灵敏度
范围
(M/Z)
分辨率
(FWHM)
扫描速度
碎片化
电离
Orbitrap (with LTQ
Thermo Scientific
Hybrid
<1
Attomole1
50–4 000
>100 000
1 spectra/s
CID, HCD, ETD, PQD
MALDI, HESI, ESI, nano, API,
APCI, APCI/APPI
XL or Velos)
TSQ Vantage
Thermo Scientific
Triple quadrupole
50*
Attomole1
10–1500
7500
5 000 units/s
CID
HESI, APCI/APPI, nano
Velos
Thermo Scientific
Linear ion trap
50*
Attomole1
50–4 000
>25 000
33 333 units/s
CID, ETD, PQD
ESI, APCI, APCI/APPI, nano
LTQ XL
Thermo Scientific
Linear ion trap
50*
Attomole1
50–4 000
>25 000
16 000 units/s
CID, ETD, PQD
ESI, APCI, APCI/APPI, nano
Xevo G2 Qtof
Waters
Quadrupole TOF
<1
Femtomole2
<100 000
>22 500
30 spectra/s
CID
ESI/APCI/ESCi, APCI, APPI/APCI, nano,
ASAP, APGC, TRIZAICTM
Xevo TQ-S
Waters
Tandem quadrupole
50*
Attomole1
2–2 048
Not provided
10 000 units/s
CID
ESI/APCI/ESCi, APCI, APPI/APCI, nano,
ASAP, APGC, TRIZAICTM
Synapt G2 HDMS
Waters
Quadrupole TOF
<1
Femtomole2
<100 000
40 000
20 spectra/s
CID
ESI/APCI/ESCi, APCI, APPI/APCI, nano,
MALDI, ASAP, APGC, TRIZAICTM
SolariX FTMS
Bruker Daltonics
FTMS
<1
Attomole3
<100 000
>1 000 000
Not provided
CID, ETD, ECD, (SORI)-CID
ESI, nano, APCI, APPI
Amazon ETD
Bruker Daltonics
Linear ion trap
50*
Attomole1
50–3 000
20 000
52 000 units/s
CID, ETD/PTR
ESI, APCI, APPI, nano, HPLC-Chip,
ESI/MALDI
micrOTOF-Q II
Bruker Daltonics
Quadrupole TOF
<2
Attomole4
50–20 000
20 000
Not provided
CID
ESI, APCI, ESI/APCI, APPI, nano, CE/MS
6 490 Triple
Agilent
Triple quadrupole
50*
Zeptomole5
10–2 000
Not provided
150 MRM/s
CID
HPLC-chip, ESI, APCI, APPI, MMI
Quadrupole
6 500 Q-TOF
Agilent
Quadrupole TOF
<2
Femtomol1
20–20 000
40 000
20 spectra/s
CID
ESI, APCI, ESI/APCI, APPI, MALDI,
HPLC-Chip
TripleTOF 5 600
ABSciex
Triple TOF
<1
Femtomol1
<40 000
40 000
100 spectra/s
CID
ESI/APCI, Turbo V, nano
Qtrap 5 500
ABSciex
Triple quadruple
100*
Femtomol1
5–1 250
Not provided
12 000 units/s
CID/ETD
APCI, Turbo V, nano, ESI/APCI, photo
Linear ion trap
100*
Femtomol1
50–1 000
9 200
20 000 units/s
注
: *
使用道尔顿为质量精度的仪器都通过
1000
的
m/z
使用转换为
p.p.m
单位
;
灵敏度使用
(1)
利血平
; (2) [Glu1]-
血纤维蛋白肽
B; (3)
泛素
; (4) BSA
消化物或
; (5)
维拉帕米来判定
;
所有的数据获取自制造商的官方网站
(
,
和
的仪器说明书或其销售代表提供的参数指标。
APGC,
常压气相色谱
(atmospheric pressure gas
chromatography); APCI,
常压化学电离化
(atmospheric pressure chemical ionization); API,
常压电离
(atmospheric pressure ionization); APPI,
常压光电离
(atmospheric pressure photoionization); ASAP,
常压固体分析探针
(atmospheric
solids analysis probe); CE/MS,
毛细管电泳质谱
(capillary electrophoresis MS); CID,
碰撞诱导解离
(collision-induced dissociation); ESCi,
电喷雾常压化学电离
(electrospray atmospheric pressure chemical ionization); ESI,
电喷雾离子
化
(electrospray ionization); FTMS
，傅里叶变换质谱
(Fourier transform MS); HCD
，高能量的
C
-陷阱解离
(higher-energy C-trap dissociation); HESI,
加热电喷雾电离
(heated electrospray ionization); MMI,
多模式电离
(multi-mode
ionization); MRM,
多反应监测
(multiple reaction monitoring); nano,
纳升喷雾
(nanospray); photo,
光电离
(photoionization); PQD,
脉冲
Q
碰撞诱导解离
(pulsed Q collision-induced dissociation); PTR,
质子转移反应
(proton transfer
reaction); SORI,
持续非共振辐照碰撞诱导解离
(sustained off-resonance irradiation)
