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Plant Gene and Trait 2012, Vol.3, No.1, 1
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to the same group.
Figure 5 Molecular evolution analyses in putative amino acid
sequence of
Goactin1
2 Discussion
Actin
with high conservatism was one of the most
antiquity and ubiquitous proteins (Meagher et al.,
1999), which was vital for plant natural morphochoresis
that action of
Actin
in deciding cell division, keeping
cytoskeleton structure, guiding cell elongation and cell
wall deposition as major component of cell
microfilaments (Zhou et al., 2001). At present,
Actin
gene had been cloned in
Arabidopsis thaliana, Oryza
sativa, Zea mays, Populus trichocarpa, Selaginella
moellendorffii, Ricinus communis, Olea europaea,
physcomitrella patens, Microul a sikkimensis,
Agropyron mongolicum, Cucumis sativus, Malus
robusta, Dunaliella salina, Helianthus annuus
etc,
based on result of GenBank data base and document
search. In Blast analyses, 84 percent homologue to
Populus trichocarpa
(XM_002311131, GENE: 7467546),
Vigna radiate
(AF143208.1) and
Betula luminifera
(FJ410442.1) was the highest in BlastN. Moreover,
the maximum number in amino acid homologue was
99 percent came from
Ricinus communis
(XP_002530-
711.1, EEF31665.1),
Solanum tuberosum
(CAA39280.1),
Gossypium hirsutum
(AAC31886.1),
Populus trichoc-
arpa
(XP_002308365.1, XP_002322664.1, ABK9278-
9.1, XP_002311167.1, EEE88534.1, XP_002316289.1,
ABK92513.1, EEF02460.1),
Caragana Korshinskii
(ACK87035.1) and
Nicotiana tabacum
(ACH69153.1,
CAA45149.1). To avoid influence of non-coding
region, BlastN analyses only with coding region
sequence (CDS) showed the highest homologue still
was 84% came from
Populus trichocarpa
(XM_00231-
1131, GENE:7467546),
Vigna radiate
(AF143208.1)
and
Betula luminifera
(FJ410442.1). It was obvious
that cDNA homologue was far less than putative
amino acid sequence. All these showed that though the
nucleotide sequence had big variations in different
plants, its amino acid sequence still kept high stability
and uniformity, which ensured plant growth and
development process on the rails.
3 Materials and Methods
3.1 Materials
Plant materials came from the early floral organ of
Ostara
(
Guzmania
cultivar).
3.2 Methods
Since 2008, we had constructed successfully full
length cDNA plasmid library of Ostara floral organ,
and obtained 1758 high quality sequences by 5'EST
sequencing to 2004 positive clones picked at random
(Liu et al., 2009). After all sequences were analyzed
by Blast, 10 EST involved in
Actin
gene were
obtained, which included three single EST such as:
|ppfca0_000981.z1.scf|, |ppfca0_000514.z1.scf|, |ppfc-
a0_000958.z1.scf| and two contigs that the first contig
inclded |ppfca0_0001_C12.ab1|, |ppfca0_001242.z1.s-
cf|, |ppfca0_000453.z1.scf| monoclone, and the second
contig inclded |ppfca0_001161.z1.scf|, |ppfca0_00124-
4.z1.scf|, |ppfca0_000774.z1.scf|, |ppfca0_001078.z1.s-
cf| monoclone.
And then, through Primer Walking sequencing to
every single EST and contig in Hangzhou Genomics
Institute, four full length cDNA sequences of
Actin
gene were obtained, one of which was analyzed and
published in the paper, the rest would be published in
other papers.
Some characters involved in cDNA sequence, amino