Basic HTML Version
Plant Gene and Trait 2012, Vol.3, No.1, 1
-
5
http://pgt.sophiapublisher.com
5
acid sequence, conservative domain and genetic
relationship, were analyzed by diversified bioinformatics
methods, for instance, prediction of amino acid sequence
by ExPASy combined with BlastP, multisequencing
comparison of nucleotide and amino acid by Blast,
estimation of protein molecular weight and isoelectric
point by ProtParam, analysis of protein secondary
structure by SPOMA, search of conservative domain
used cdart program (NCBI), prediction of protein
tertiary structure by ESyPred 3D and phylogenetic
tree analyse by MEGA 4.0 combined with ClustalX
(1.81) software.
Author Contributions
Jianxin Liu was deviser, superintendent and principal transactor;
Yaying Ge, Danqing Tian and Zhi Zhang participated in
experimental design, test result analysis and writing of the
preliminary draft; Huaqiao Ding, Fuquan Shen and Weiyong
Wang provided test materials and analyzed data. All authors
had read and approved the final version.
Acknowledgements
This research was supported by the great science and technology
special project of Zhejiang Province (No.2009C12095), the
technological innovation ability promoting project in Zhejiang
Academy of Agricultural Sciences.
References
Combet C., Blanchet C., Geourjon C., and Deléage G., 2000, NPS@:
network protein sequence analysis, Tibs., 25(3): 147-150
Hussey P.J., Ketelaar T., and Deeks M.J., 2006, Control of the
Actin
cytoskeleton in plant cell growth, Annu. Rev. Plant Biol., 57: 109-125
Kost B., Mathur.J, and Chua N.H., 1999, Cytoskeleton in plant development,
Curr. Opin. Plant Biol., 2(6): 462-470
Lambert C., Leonard N., de Bolle X., and Depiereux E., 2002, ESyPred3D:
Prediction of proteins 3D structures, Bioinformatics, 18(9): 1250-1256
Liu J.X., Shen F.Q., Tian D.Q., Zhang Z., Ge Y.Y., Shen X.L., Yu X.Y., and
Wang W.Y., 2009,Construction of full-length cDNA library from floral
organ and EST analysis in Guzmania, Fenzi Zhiwu Yuzhong
(Molecular Plant Breeding), 7(6): 1137-1143
Liu J.Y., 2003, Real-Time PCR technique and its application in
quantification of plant nucleic acid molecules, Acta Botanica Sinica,
45(6): 631-637
Ma X.F., Yu C.M., Tang S.W., Zhu A.G., Wang Y.Z., Zhu S.Y., Liu J.X., and
Xiong H.P., 2010, Cloning and tissue expression of
Actin
1 gene in
different fiber development phases of ramie [
Boehmeria nivea
(Linn.)
Gaud], Zuowu Xuebao (ACTA AGRONOMICA SINICA), 36(1):
101-108
Marchler-Bauer A., and Bryant S.H., 2004, CD-Search: protein domain
annotations on the fly, Nucleic Acids Res., 32(W): 327-331
Marchler-Bauer A., Anderson J.B., Chitsaz F., Derbyshire M.K.,DeWeese-Scott
C., Fong J.H., Geer L.Y., Geer R.C., Gonzales N.R., Gwadz M., He S.,
Hurwitz D.I., Jackson J.D., Ke, Z., Lanczycki C.J., Liebert C.A., Liu C.,
Lu F., Lu S., Marchler G.H., Mullokandov M., Song J.S., Tasneem A.,
Thanki N., Yamashita R.A., Zhang D., Zhang N., and Bryant S.H., 2009,
CDD: specific functional annotation with the Conserved Domain
Database, Nucleic Acids Res., 37(D): 205-210
Meagher R.B., McKinney E.C., Kandasamy M.K., 1999, Isovariant
dynamics expand and buffer the responses of complex systems: The
diverse plant
Actin
gene family, The Plant Cell, 11: 995-1006
Yan L.F., and Shi D.Q., 1963, The presence of a contractile protein in higher
plants, Shenghua Yu Shengwu Wuli Xuebao (Acta Biochim. Biophys.
Sin.), 3(4): 490-495
Yang F.L., Yue H., Xie X.L., and Jia W.X., 2007, Development of real-time
PCR assay for duck GAPDH gene, Xinan Minzu Daxue Xuebao •
Ziran Kexue Ban (Journal of Southwest University for Nationalities
⋅
Natural Science Edition)), 33(1): 92-95
Zhang S.B., and Liu G.Q., 2006, Research advances in plant actin isoforms,
Zhiwuxue Tongbao (Chinese Bulletin of Botany), 23(3): 242-248
Zhou H.F., Zhao W.L., and Yan L.F., 2001, Cloning and sequence analysis
of
Actin
gene from
Magnolia denudata
Desr., Nongye Shengwu Jishu
Xuebao (Journal of Agricultural Biotechnology), 9(3): 274-278
Zhu H., Li S., Zhao R.Q.,Wu Y.S., 2006, Cloning and sequencing of Panax
notoginseng GAPDH, Xibei Zhiwu Xuebao (Acta Bot.Boreal.-Occident. Sin.),
26(7): 1316- 1319