Page 11 - mpbcn2013no30

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张瑛等
:
植物脯氨酸测定方法优化及其应用
1221
1
不同浓度脯氨酸标准溶液配制方法
Table 1 Compound method of standard solution of proline under different concentrations
脯氨酸浓度
(μg/mL)
The concentrations of proline (μg/mL)
脯氨酸体积
(mL)
The volumes of proline (mL)
(mL)
The volumes of water (mL)
0
取浓度为
10 μg/mL
的脯氨酸标准溶液
The standard solution of proline with 10 μg/mL
concentration
0.00
2.00
0.5
0.10
1.90
1
0.20
1.80
2
0.40
1.60
4
0.80
1.20
6
1.20
0.80
8
1.60
0.40
10
2.00
0.00
15
取浓度为
50 μg/mL
的脯氨酸标准溶液
The standard solut
ion of proline with 50 μg/mL
concentration
0.60
1.40
20
0.80
1.20
25
1.00
1.00
30
1.20
0.80
35
1.40
0.60
40
1.60
1.40
50
2.00
0.00
60
取浓度为
100 μg/mL
的脯氨酸标准溶液
The standard solution of proline with 100 μg/mL
concentration
1.20
0.80
75
1.50
0.50
1
脯氨酸标准曲线
: A: 0
-
75 µg/mL; B: 0
-
10 µg/ mL; C: 15
-
35 µg/mL; D: 40
-
75 µg/ mL
Figure 1 Proline standard curve
Note: A: 0
-
75 µg/mL; B: 0
-
10 µg/ mL; C: 15
-
35 µg/mL; D: 40
-
75 µg/ mL