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宋云等：金花茶植物物种鉴定的
dCAPS
标记开发及其应用
1192
图
1
金花茶与其它近源种的
GBSSI
基因部分序列比对分析
注
:
方框标注为本实验中所用
SNP
位点
;
下划线序列为
dCAPS
引物设计所用目标序列
Figure 1
Partial sequence alignment of the
GBSSI
gene region showing the Single Nucleotide Polymorphisms (SNPs) between
C.
nitidissima
and other closely-related species
Note: The box shows the SNPs used in this study; The underlined sequences were entered to the dCAPS Finder 2.0 program for
dCAPS primer design
图
2
金花茶
dCAPS
引物设计
注
:
下划线的大写字母表示
1
个
SNP;
下划线的小写字母表示引物中设置的错配碱基
Figure 2 The design of dCAPS primer
Note: The capital letter with an underline indicates a SNP and the lowercase with an underline indicates a mismatch base in a dCAPS
primer;
C. nitidssiam
is designated as wild-type and other species as mutant