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分子植物育种(网络版), 2012 年, 第 10 卷, 第 1145- 1150 页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1145- 1150
http://mpb.5th.sophiapublisher.com
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表 1 引物序列
Table 1 Primer sequences
序号 正向引物 反向引物
No. Forward primers Reverse primers
1 5'- TTTGTCCCTATTGCTCAGGG-3' 5'- CCGAGAACGTCTTCTCCTTG- 3'
9 5 -ACCTTCCGTCTCTGGGTCTT -3' 5'- TTTTGAACGAGGTGGAGGAC-3'
26 5'- ATAACAACAACCTGCCCGAG-3' 5'- GAAGCACAAGAACAGGGCTC- 3'
图 2 引物 1 大白菜杂交种纯度鉴定
注: 1 号, 2 号, 3 号, 5 号, 6 号, 9 号品种的大白菜杂交种种子纯度鉴定结果, 1, 2, 3 分别为 1 号品种大白菜的母本, 杂交种, 父
本; 4, 5, 6 分别为 2 号品种大白菜的母本, 杂交种, 父本; 7, 8, 9 分别为 3 号品种大白菜的母本, 杂交种, 父本; 10, 11, 12 分别
为 5 号品种大白菜的母本, 杂交种, 父本; 13, 14, 15 分别为 6 号品种大白菜的母本, 杂交种, 父本; 16, 17, 18 分别为 9 号品种
大白菜的母本, 杂交种, 父本
Figure 2 Results of purity identification of Chinese cabbage hybrids by primer No.1
Note: Results of purity identification of Chinese cabbage of No.1, 2, 3, 5, 6, 9 hybrids, 1, 2, 3 were maternal parent, hybrid, paternal
parent of No.1 hybrid respectively; 4, 5, 6 were maternal parent, hybrid, paternal parent of No.2 hybrid respectively; 7, 8, 9 were
maternal parent, hybrid, paternal parent of No.3 hybrid respectively; 10, 11, 12 were maternal parent、hybrid 、paternal parent of No.5
hybrid respectively; 13, 14, 15 were maternal parent、hybrid 、paternal parent of No.6 hybrid respectively; 16, 17, 18 were maternal
parent, hybrid, paternal parent of No.9 hybrid respectively
图 3 引物 9 大白菜杂交种纯度鉴定
注: 1 号, 3 号, 4 号, 5 号, 7 号, 8 号品种的大白菜杂交种种子纯度鉴定结果, 1, 2, 3 分别为 1 号品种大白菜的母本, 杂交种, 父
本; 4, 5, 6 分别为 3 号品种大白菜的母本, 杂交种, 父本; 7, 8, 9 分别为 4 号品种大白菜的母本, 杂交种, 父本; 10, 11, 12 分别
为 5 号品种大白菜的母本, 杂交种, 父本; 13, 14, 15 分别为 7 号品种大白菜的母本, 杂交种, 父本; 16, 17, 18 分别为 8 号品种
大白菜的母本, 杂交种, 父本
Figure 3 Results of purity identification of Chinese cabbage hybrids by primer No.9
Note: Results of purity identification of Chinese cabbage of No.1, 3, 4, 5, 7, 8 hybrids, 1, 2, 3 were maternal parent, hybrid, paternal
parent of No.1 hybrid respectively; 4, 5, 6 were maternal parent, hybrid, paternal parent of No.3 hybrid respectively; 7, 8, 9 were
maternal parent, hybrid, paternal parent of No.4 hybrid respectively; 10, 11, 12 were maternal parent, hybrid, paternal parent of No.5
hybrid respectively; 13, 14, 15 were maternal parent, hybrid, paternal parent of No.7 hybrid respectively; 16, 17, 18 were maternal
parent, hybrid, paternal parent of No.8 hybrid respectively
父本和母本互补的共显性带型，并对所筛选引物进
行田间验证，验证结果为大白菜的 EST-SSR 分子标
记方法与田间形态观察方法纯度鉴定结果一致，即
所建立的技术可用来鉴定大白菜杂交种的种子纯
度。并且该技术在不计算 DNA 提取过程耗时的情
况下，PCR 耗时 2 h，EST-SSR 分析 60~90 min，所
以本研究的检测方法在 4 h 之内即可完成种子纯度
鉴定工作，具有快速、低成本、操作方便等优势。