Basic HTML Version
分子植物育种
(
网络版
), 2011
年
,
第
9
卷
,
第
1471
-
1478
页
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1471
-
1478
http://mpb. chinese.sophiapublisher.com
1476
图
4
植物淀粉合成酶的进化关系
注
:
利用
CLUSTAL X (Ver. 1.83)
软件对植物淀粉合成酶氨基酸序列进行多序列排列
;
用软件
MEGA 4.0
构建邻接
(neighbor-joining, NJ)
树
;
选择
Poisson Correction(
泊松修正
)
这一模型
;
分析时不考虑插入和缺失
,
节点支持率使用
1 000
次
Bootstrap
检验
;
物种名称及蛋白质序列登陆号如下
:
水稻
: rice (
Oryza sativa
) GBSS
Ⅰ
, ACJ86365; GBSS
Ⅱ
, BAF26592; SS
Ⅱ
c, AAL16661; SS
Ⅱ
a, AAK64284; SS
Ⅱ
b, AAK81729; SS
Ⅲ
a, AAL40942; SS
Ⅲ
b, AAM49811; SS
Ⅳ
a, AAQ82622; SS
Ⅳ
b,
AAQ82623;
小麦
: wheat (
Triticum aestivum
) GBSS
Ⅰ
; BAA77351; GBSS
Ⅱ
, SSIa, CAB99209; SS
Ⅱ
a, CAB86618; SS
Ⅱ
b, SSIIIa,
AAF87999; SS
Ⅲ
b, ABY56823; SS
Ⅳ
, AAK97773;
大麦
: barley (
Hordeum vulgare
) GBSS, AAM74052;
玉米
: maize (
Zea mays
)
SS
Ⅱ
a, AAS77569; SS
Ⅰ
, ABQ23357; GBSS
Ⅰ
, AAF79205; SS
Ⅲ
b, ABP35815;
高粱
: sorghum (
Sorghum bicolor
), SS
Ⅱ
a,
ACC86844; SS
Ⅱ
b, ACC86845; SS
Ⅱ
c, ABY27640; SS
Ⅲ
b, ACC86847;
木薯
: cassava (
Manihot esculenta
) SS
Ⅰ
, ABV25893;
蚕
豆
: bean (
Phaseolus vulgaris
) SS
Ⅰ
, BAD18845; SS
Ⅱ
, BAD18846; SS
Ⅲ
, BAF49176; GBSS, ABU87173;
小米
: Italian millet
(
Setaria italica
) GBSS, BAC06486;
豌豆
: pea (
Pisum sativum
) GBSS, CAC69955; SS, CAA61268
Figure 4 Phylogenetic tree showing the relationship of plant starch synthases based on their deduced amino acid sequences
Note: The MEGA 4.0 and CLUSTAL X (Ver. 1.83) program were employed to align the amino acid sequences of starch synthases;
The tree was constructed using the neighbour-joining method and poisson correction model and confidence limits to branch points in
the tree were assigned by bootstrapping the alignment with a random number generator seed of 111 and 1 000 trials; Genbank
accession numbers of the starch synthases shown in the tree are as follow; Rice (
Oryza sativa
) GBSS
Ⅰ
, ACJ86365; GBSS
Ⅱ
,
BAF26592; SS
Ⅱ
c, AAL16661; SS
Ⅱ
a, AAK64284; SS
Ⅱ
b, AAK81729; SSIIIa, AAL40942; SS
Ⅲ
b, AAM49811; SS
Ⅳ
a,
AAQ82622; SS
Ⅳ
b, AAQ82623; wheat (
Triticum aestivum
) GBSS
Ⅰ
; BAA77351; GBSS
Ⅱ
, SS
Ⅰ
a, CAB99209; SS
Ⅱ
a, CAB86618;
SS
Ⅱ
b, SS
Ⅲ
a, AAF87999; SS
Ⅲ
b, ABY56823; SS
Ⅳ
, AAK97773; barley (
Hordeum vulgare
) GBSS, AAM74052; maize (
Zea mays
)
SSIIa, AAS77569; SS
Ⅰ
, ABQ23357; GBSS
Ⅰ
, AAF79205; SS
Ⅲ
b, ABP35815; sorghum (
Sorghum bicolor
), SS
Ⅱ
a, ACC86844; SS
Ⅱ
b, ACC86845; SS
Ⅱ
c, ABY27640; SS
Ⅲ
b, ACC86847; cassava (
Manihot esculenta
) SS
Ⅰ
, ABV25893; bean (
Phaseolus vulgaris
)
SS
Ⅰ
, BAD18845; SS
Ⅱ
, BAD18846; SS
Ⅲ
, BAF49176; GBSS, ABU87173; Italian millet (
Setaria italica
) GBSS, BAC06486; pea
(
Pisum sativum
) GBSS, CAC69955; SS, CAA61268