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张如华等
, 2011,
柽柳
EST-SSRs
标记开发与群体检测
,
分子植物育种
Vol.9 No.38 (doi: 10.5376/mpb.cn.2011.09.0038)
1293
表
2
柽柳属植物
EST-SSR
主要类型及频率
Table 2 Main types and frequency of EST-SSR in
Tamarix
spp.
重复单元核苷酸数
Nucleotide Number of repeat
重复序列
SSR motif
SSRS
数量
Number of SSR
总数
Total
出现频率
(%)
Frequency (%)
二核苷酸
Dinucleotide
AC\TG
3
3
6
2.42
AG\TC
10
9
19
9.22
AT\TA
11
13
24
11.65
CT\GA
11
7
18
8.73
三核苷酸
Trinucleotide
CTC\GAG
3
2
5
2.42
AAT\TTA
6
9
15
7.28
ACG\TGC
1
2
3
1.45
AGA\TCT
1
2
3
1.45
AGG\TCC
2
3
5
2.42
ATA\TAT
2
4
6
2.91
ATT\TAA
2
2
4
1.94
CTT\GAA
3
2
5
2.42
GGC\GCC
1
3
4
1.94
AAG
6
2.91
TGG
4
1.94
ATG
3
1.45
表
3
柽柳中
14
个
EST-SSRs
引物的扩增特性
Table 3 The characteristics of 14 EST-SSRs primer amplification in
T. chinensis
L.
引物号
/
登录号
No./Accession No.
引物序列
(5'
-
3')
Primer sequences (5'
-
3')
重复单元
Repeat motif
预计片段大小
(bp)
Expected size (bp)
等位基
因
A
实际杂合
度
Ho
期望杂合
度
He
Estssr
-
1/CF199044
F:GGGTGGACGACTTGGC
(ATT)
6
170
3
0.6256
0.6952
R:CGTGCGTGCGTATCAA
Estssr
-
2/EH051607
F:AACCACCGATACATACAGA
(ATCT)
5
235
6
0.6986
0.7469
R:TGACAAATGACCTCCAAG
Estssr
-
3/EH053495
F:GCGGTGCTTCCTTCATCT
(TCC)
6
205
5
0.4429
0.4241
R:CAAATGGGAGTGATGATGG
Estssr4/CV790971
F:GGGCAATGTTTCCGATTC
(ATGAAA)
4
290
5
0.6256
0.6184
R:AGGCGACTGTAGCCAAGC
Estssr
-
5/EG967460
F:CATTTAGGACGAAACGAA
(TC)
9
187
3
0.3516
0.5008
R:CACGAGTGGCTTGACAGA
Estssr
-
6/AB298390
F:GTCCGTCGCTCTTGTATTT
(AC)
9
189
6
0.6575
0.7840
R:GGTGATTGAAGCCATTGTTAT
Estssr
-
7/EH051950
F:CCAAATCCATCTTTCTC
(AAT)
7
248
3
0.3740
0.4029
R:CCTCTTCCAATCTGCT
Estssr
-
8/EH050298
F:TACCACGAGCAAACCAA
(TGAAGA)
3
232
4
0.4566
0.5601
R:GCGACTGTAGCCAAGC
Estssr
-
9/EG973825
F:TATCTCCATCCCAACAGT
(AT)
9
270
5
0.6256
0.6964
R:CGGCACGAGGCGGATA
Estssr
-
10/EH056738 F:ATAGGCGAATGGGTAC
(ATT)
7
190
5
0.6986
0.7458
R:GTCTCAGCCAATGGAG
Estssr
-
11/EH048358 F:GCCCTTTCTAACCATC
(AC)
8
354
5
0.4429
0.4252
R:GTCCAGAGGAGCAACC
Estssr
-
12/EH056853 F:ACTGACTGAACGTGGCG
(AAT)
7
320
4
0.6256
0.6173
R:GGCTGCGGCTTCTACT
Estssr
-
13/CF199807 F:TAACAATCGCAATCCT
(ATTAC)
4
410
6
0.3516
0.5060
R:AACTGTAATGACCCACA
Estssr
-
14/CV795002 F:TACAGGGAGAACATAGGG
(AG)
13
259
3
0.6575
0.7820
R:CGAGAAGGGAGGACAG