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Table of Contents
Table of Contents
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Cover 1 -
Copyright 2 -
Contents 3 -
Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T1 Generation 4 -
Abstract 4 -
Keywords 4 -
Background 4 -
1 Results and Analysis 5 -
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1.1 The effects of explant culture time on the shoot tip growth status 5 -
1.2 The effect of bacterium infection time on the shoot apex growth status 5 -
1.3 The effect of co-culture time on shoot apex growth status 5 -
1.4 Obtaining transgenic SNC1 cotton 6 -
1.5 PCR detection of transgenic SNC1 cotton plants in T1 generation 6 -
1.6 T1 generation of transgenic cotton plants identified by RT-PCR 7 -
1.7 Identification of disease resistance of transgenic cotton T1 generation plants by in vitro infection 7
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2 Discussion 7 -
3 Materials and Methods 8 -
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3.1 Receptor materials 8 -
3.2 Plasmids and Agrobacterium strains 8 -
3.3 Reagents 8 -
3.4 Agrobacterium-mediated genetic transformation 9 -
3.5 Molecular detection of transgenic plants 9 -
3.6 T1 generation of transgenic plants identified by RT-PCR 9 -
3.7 Disease resistance of transgenic plants identified by inoculation 9
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Acknowledgements 9 -
References 10 -
Back cover 12