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Cotton Genomics and Genetics 2012, Vol.3, No.1, 1-7
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Research Report Open Access
Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene
and Resistance to Cotton FusariumWilt in T1 Generation
Jiangrong Lei , Dongmei Wang , Lin Shao , Xiaowei Wei , Leping Huang
Institute of Nuclear and Biological Technology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091
Corresponding authors email: wdm0121@yahoo.com.cn; Authors
Cotton Genomics and Genetics, 2012, Vol.3, No.1 doi: 10.5376/cgg.2012.03.0001
Received: 18 Jan., 2012
Accepted: 02 Jul., 2012
Published: 09 Jul., 2012
This article was first published in Molecular Plant Breeding (2010, Vol.8, No.2, 252-258) in Chinese, and here was authorized to translate and publish the paper
in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original work is properly cited.
Preferred citation for this article:
Lei et al., 2012, Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T1 Generation,
Cotton Genomics and Genetics, Vol.3, No.1 1-7 (doi: 10.5376/cgg.2012.03.0001)
Abstract Using shoot apexes from the upland cotton varieties, named Zhong35 and Junmian No.1 as explant, the SNC1, suppressor
of npr1- 1constitutive cloned from Arabidopsis thaliana , was introduced into cotton genome by Agrobacterium-mediated
transformation with the optimized transformation conditions including culture status of the explant, infection time and co-culture
time. The results showed that the transformation procedure for high transformation rate was as follows, in vitro cuture the explants
one day, infecting 20 min and co-culturing two days. PCR and RT-PCR identification of T0 and T1 regeneration plants revealed that
the SNC1 gene was successfully integrated into cotton genome and expressed in the transgenic plant. Furthermore, comparing with
non-transgenic plants, the T1 generation transgenic plants acquired the significant resistance to the inoculation of with the Fusarium
oxysporum f.sp. Vasinfectum by the validation of the root-dipping method, indicating that the SNC1 should facilitate the resistant to
cotton Fusarium wilt.
Keywords Upland cotton; SNC1; Agrobacterium-mediated transformation; Fusarium wilt
Background
Cotton Fusarium wilt is a destructive wilt disease in
cotton production. The right way controlling cotton
Fusarium wilt is to adopt the resistant germplasms and
to develop resistant cotton varieties. However, it is
not yet clear about the genetic rules of cotton
disease-resistant varieties, in fact there was a dearth
of materials for resistance to Fusarium wilt in cotton
germplasms. Therefore, it is difficult to breed cotton
varieties resistant to Fusarium wilt through
conventional breeding methods. In order to solve the
problem that could not be solved with the conventional
breeding methods, the best method is to directly
introduce the required disease resistant gene into cotton
genome to enhance the resistance to cotton wilt.
Since the advent of the first case of genetically
modified crops, crop genetic transformation technology
has been flourishing. Cotton being one of the popular
fiber crops is also being taken seriously in the
application of genetic transformation technology. So
far, the Agrobacterium-mediated approach compared
with other methods of gene transformation possesses
advantages with mature procedure, simple mani-
pulation, clear transformation mechanism and high
eff iciency, etc . Transgenic methods can be in
accordance with the wishes of the people not only to
improve crop, but also to shorten the breeding periods
3 or 4 years than that of the conventional hybrid
breeding methods. Up to now, Gastrodia antifungal
protein gene, tobacco beta-1, 3 glucan gene and bean
chitinase gene and so on have been successfully
introduced into the cotton by using transgenic pathway,
which have developed transgenic cotton lines with
resistance to Verticillium wilt (Wu et al., 2004; Wang
et al., 2004; Cui et al., 2002; Le et al., 2002; Tsai et al.,
2000). It is no doubt that the genetic engineering
exhibits its broad prospects for the disease resistant
improvement in cotton breeding program.
So far, there are more studies on the system acquired
resistance (SAR) in model plant Arabidopsis. Once
plants are infected by the pathogen, the local
hypersensitive resistance (HR) will generate some