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分子植物育种
(
网络版
), 2010
,
8
,
1
Fenzi Zhiwu Yuzhong (Online), 2010, Vol.8, No.1
http://mpb.
5th
.sophiapublisher.com
10
页,第
4
于另一个
EST-PCR
产物而被测序。
3
P
2
中片段长度杂合的标记
EUCeSSR0946 (13
)
的部分序列及其与源
EST
的对比
: A: PCR
产物直接测序
; B: 16
PCR
产物混合克隆中的测序
; C: 32
PCR
产物混合克隆中的测序
; Seq1
对应的测序峰一
般稍高于
Seq2;
灰色阴影部分的序列为
EST-SSR
的重复单位
;
下划线表示重复单位后的对应序列
Figure 3 Partial sequences of the lengthheterozygous EST-SSR marker EUCeSSR0946 (No. 13) within P
2
and their comparison to the
original EST
Note: A: Direct PCR product sequencing; B: pool cloning with PCR products of 16 EST-SSRs; C: Pool cloning with PCR products of
32 EST-SSRs; Seq1 corresponding mostly to the higher traces in the sequencing profile and Seq2 to the lower ones; The bases in grey
shadow show the repeat motifs of the EST-SSR marker within P
2
, while those underlined in (A) show the identical bases following
the repeats