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10
页，第
9
页
序号
No.
EST-SSR
标记
EST-SSR locus
EST
号
GenBank
accession
引物对
(5'
-
3')
Primer pair (5'
-
3')
EST
设计长度
(bp)
EST t arget size
(bp)
PCR
产物长
度
a
(bp)
PCR product
size
a
(bp)
EST
的重复单位
Repeats
in EST
测序确认的
P
2
的重复单位
Repeats in P
2
as revealed in sequencing
序列一致
性
(%)
Sequence
identity (%)
PCR
产物直接测序
b
Direct PCR product
sequencing
b
混合克隆测序
c
Pool cloning of
PCR products
c
15
EUCeSSR1014
CU395155
F:GGGACTGAGCTCTGATCCTACT
R:GACCGCTGGTACTTGCTG
235
396/402
(AG)
3
AA(AG)
3
G
AAGCAAGAGA
T(AG)
15
GAAG
AC(AG)
9/12
:
[24/8/32]
97.42
16
EUCeSSR1083
CU403250
F:CGGGGTCTCTCGAAGCGTCT
R:CCGACGGCATCGTAAACC
314
435
(GGAAGC)
3
(GGAAGC)
3
:
[24/16]
97.45
17
EUCeSSR1136
CU396171
F:GATGGCAGGACATAAAGA
R:GCTAAACAAACCAGGAAA
373
490/492
(GA)
8
AA(GA)
9
[(G/A)A](GA)
5
[(G/A)A](GA)
20/21
98.79
18
EUCeSSR1091
CU404650
F:GTTCATCCGCCCTTACAG
R:ACCCTTGCTCCTTCTTCG
402
∼
490
(TGTTT)
3
+T
14
G
3
TGTT(TGTTT)
2
+
(T
9
G
17
)/(T
8
G
12
A
3
G
4
)
97.51
19
EUCeSSR0894
EW688426
F:AGTCCTTCAGCAGGTTCA
R:CCGCCGGTATAGTAGTTG
145
518
(CAC)
6
(CAC)
6
92.78
20
EUCeSSR0961
CU396545
F:CCCCTTCACTGCTCTTCT
R:ACCAACCGCGAGTATGTC
242
547
(GCT)
6
(GCT)
n/(n+1)
(GCT)
3
[(G/A)CT]
(GCT)
2/3
: [32/24/16]
98.76
21
EUCeSSR1087
CU403690
F:TGAGGCACAACAAGGTAA
R:CTGGCTGTAGATTGAAGAGT
439
∼
650
(AGC)
10
22
EUCeSSR1054
CU398509
F:CTTCCTCGTCTTCTTCTGC
R:TAAGGTCACCCAAGTTCTG
241
665/671
(CTG)
2
(CCG)
(CTG)
6
:
(CTG)
(n+3)/(n+5)
(CTG)
1/2
(CCG)
(CTG)
9/10
: [32/16/8]
99.59
23
EUCeSSR1122
GO248349
F:ACAACAATCTTGCCTTCT
R:TCACAATAAACGAGTCCC
361
687
(CTG)
6
(CTG)
6
(CTG)
6
: [32/24/16]
99.59
24
EUCeSSR1011
CU396518
F:GCAGAGGAGCGGAAACTA
R:GCTACTTTGGGTGTGTTCT
440
∼
850
(TC)
9
25
EUCeSSR1150
CU397973
F:ACCCTCCTCCTCCGTATT
R:CACATCCCATCTCCTTCC
440
∼
850
(TC)
6
26
EUCeSSR0970
CU402791
F:GGCCCGCCTTTCCAATTC
R:TTGTCTCGGTTGGGTTCT
225
997
(TC)
10
(TC)
10
: [32/16/8]
91.11
27
EUCeSSR0914
CU397331
F:CCGGGGGCTTTGCTTTCA
R:TACCTCCATTGCTTCCTT
220
∼
1200
(AG)
10
(AG)
10
: [32]
96.36
28
EUCeSSR1069
CU400565
F:ACCTCCATCCTAAGACGA
R:ATTCCCAGAAGACCCATT
318
1175
(GA)
5
CA(GA)
4
(CA)(GA)
5
CA
(GA)
3
(GA)
15/16
(GA)
15
: [32/24]
97.36
29
EUCeSSR1102
EW688317
F:TTCTTTCTTGGGTTCAGT
R:ACAAGGCAATGCTTTCTC
371
∼
1300
(GG)
2
(AG)
7
AA(A
G)
3
GG(GA)
10/12
:
[32/24/16]
98.36