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共
10
页，第
8
页
表
1
候选
32
个桉树
EST-SSR
的引物以及对细叶桉
(P
2
)
的
PCR
序列确认
Table 1 Primer pairs and sequence validation for the 32 candidate EST-SSRs of
Eucalyptus
序号
No.
EST-SSR
标记
EST-SSR locus
EST
号
GenBank
accession
引物对
(5'
-
3')
Primer pair (5'
-
3')
EST
设计长度
(bp)
EST t arget size
(bp)
PCR
产物长
度
a
(bp)
PCR product
size
a
(bp)
EST
的重复单位
Repeats
in EST
测序确认的
P
2
的重复单位
Repeats in P
2
as revealed in sequencing
序列一致
性
(%)
Sequence
identity (%)
PCR
产物直接测序
b
Direct PCR product
sequencing
b
混合克隆测序
c
Pool cloning of
PCR products
c
1
EUCeSSR1085
CU403532
F:GTTGTACTCTGCCCCTAA
R:CAGCCTCGGATTGTTTCA
118
111 (113)
(CT)
8
(CT)
6
(CT)
5
: [16]
97.30
2
EUCeSSR1078
CU402447
F:GTGGCATTCACAAGAGCA
R:GGAAGCCTTTCGGGATAA
126
129
(CAA)
5
(CAA)
5
(CAA)
5
: [32]
100.00
3
EUCeSSR0984
CU395760
F:TCTTACGCACCGTCCTCT
R:AGCCATTTCGCTCCTTCC
130
139
TCGTCA
(TCG)
6
(TCG)
(2+n)
T(C/A)T
(TCC)
2
(TCG)
8
(TCT)
(TCC)
2
: [32]
97.69
4
EUCeSSR1110
GO247750
F:ATCTCCGCACCCACTCTT
R:TGTCATTCCTCGTTCAGC
185
173
(CCAATC)
3
CCAATT
CCAATT:
[24/16/8]
90.23
5
EUCeSSR0925
CU402880
F:GAAGGCAGAGGCTTACGA
R:CTTATTTTTCCTCCCCCCAA
222
209
(ATC)
11
(ATC)
6
99.32
6
EUCeSSR1135
CU402600
F:GAACTGCTGCTGCCGAATG
R:TGAAGCCCAAGGAAATGC
217
205
(CT)
14
(CT)
8
(CT)
8
: [32/24/16/8]
100.00
7
EUCeSSR0980
EW688281
F:TGACCCTTCGGCTTCCTT
R:GGGCTCGCCAATCTCCAT
217
214
(GAG)
6
(GAG)
5
(GAG)
5
: [24]
99.07
8
EUCeSSR0985
CU396018
F:CTTGTTCGGGTTTCTTTCA
R:CACTGAGTAACAGGCAAG
264
242/244/246
(AG)
20
(AG)
(8+n)/(9+n)
(AG)
9/10
:
[32/24/16/8]
99.19
9
EUCeSSR0955
GO248042
F:CATTGGAGTAGCACGAGAA
R:ACTCCATACTCTCTGTGTCTCT
257
263
(GAC)
7
+ (GA)
7
(GAC)
7
+ (GA)
10
99.04
10
EUCeSSR1046
CU396352
F:TCAAACCCAGCGATACCC
R:AGCCCGATGATCCAAATAA
264
266
(TGC)
5
(TGC)
5
98.61
11
EUCeSSR1049
CU397549
F:GGTGATGAGGTGGGACAG
R:GAGTGGAGGTTTCTTATGG
294
312/314
(GA)
7
(GA)
11/12
94.76
12
EUCeSSR1075
CU402010
F:TGCGTGTTTGCTCAGGAT
R:TGACATTGCCCCTTCGAT
379
368
(GA)
18
(GA)
5
(GGGA)
2
GAGGACAA
(GA)
5
(GGGA)
2
GAG
GACAA: [32]
96.73
13
EUCeSSR0946
CU403671
F:ACTCCGTCTACATAGCATC
R:CCAAGACCACCAAACATA
372
366/370
(TC)
10
(TC)
7/9
(TC)
7/9
: [32/16]
98.92
14
EUCeSSR1002
CU395531
F:GAGGAGGATGACGAGATGAG
R:CGCAGATAAGTAAGAAGGCTAA
370
370
(CAC)
5
(CAC)
5
93.15