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10
页，第
10
页
序号
No.
EST-SSR
标记
EST-SSR locus
EST
号
GenBank
accession
引物对
(5'
-
3')
Primer pair (5'
-
3')
EST
设计长度
(bp)
EST t arget size
(bp)
PCR
产物长
度
a
(bp)
PCR product
size
a
(bp)
EST
的重复单位
Repeats
in EST
测序确认的
P
2
的重复单位
Repeats in P
2
as revealed in sequencing
序列一致
性
(%)
Sequence
identity (%)
PCR
产物直接测序
b
Direct PCR product
sequencing
b
混合克隆测序
c
Pool cloning of
PCR products
c
30
EUCeSSR1060
CU398944
F:CTCAGCTTCCGTATCTTG
R:TTGATTACTGCGATTTAGAG
420
1274
GG(GA)
7
(GG)
2
(GA)
13
: [24]
98.57
31
EUCeSSR1034
CU399256
F:CGGGGGCGTTTCGGAGATTT
R:CCACTCTGACAGCCCTTT
240
1450
(AGAAG)
3
97.62
(
部分序列
)
32
EUCeSSR1028
GO248406
F:GCCATTGGGTATTCTTCT
R:CTCGCTTCCTCTTTCACT
299
1500
(GA)
7
注
: a : P CR
产物长度优先根据克隆测序的结果确定
,
次则根据
PCR
产物直接测序与源
EST
的比对结果推测
,
测序未成功者根据图
1
确定
; b: PCR
产物直接测序中
,
因部分
SSR
的重复单位靠近
(
前向
)
测序引物
,
只有部分重复单位可见
, n
表示未知的重复单位数
; c:
混合克隆测序中
,
中括号内数字表示成功测序的混合克隆的
PCR
产物数量
Note: a: PCR product size was determined sequentially with pool cloning of PCR products, direct sequencing of PCR products or manually estimated from Figure 1; b: n denotes an
unknown number of SSR motif repeats missed in sequencing due to the closeness of SSR motifs to the (forward) sequencing primer si te; and c: Numerals in the brackets show the
number of EST-SSRs pooled for cloning in which the marker was successfully sequenced