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International Journal of Molecular Veterinary Research
2013, Vol.3, No.2, 4
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5
higher number was retrieved from right ovary under
slicing technique (Figure 2).
Figure 1 Oocyte recovery
Figure 2 Grade of oocytes
The yield of grade A oocytes was not significantly
differed between techniques except in slicing, The
retrieval of grade B oocyte was significantly higher in
dissection and slicing techniques rather than aspiration
in the left ovary, while, significantly higher number
was retrieved in slicing technique. The retrieval of
oocytes of grades C and D significantly higher in
slicing techniques only, from both left and right
ovaries (Figure 3).
Figure 3 Percent yield of different grade of oocyte
The present yield of oocyte was significantly higher in
slicing (50.57) followed by dissection (37.52) and
aspiration (20.70) techniques (Figure 3). However,
there was no like wise, significantly higher per cent of
A, B, C and D grade oocytes were retrieved in slicing
techniques when compared to aspiration and
dissection techniques.
Lorenzo et al. (1999) and recorded similar trend in per
cent of oocyte retrieval Kumar et al. (2000), but vice
versa was noticed by Bonde et al. (2000).
1.2 Percent yield of oocytes
In the present study, B grade oocytes were retrieved to
a greater (per cent) by aspiration (12.53), dissection
(37.07) and slicing (50.57), while the recovery of
grade D was found lowest by all the techniques. The
aspiration technique also yielded grade A oocytes to a
greater extent. The grade C oocytes were recovered to
a greater extent by slicing technique. The overall yield
was highest with slicing technique. The oocytes
remain firmly attached to the small and medium sized
follicles before cumulus expansion and cannot be
aspired, but can be easily recovered from the small
follicles when the slicing method is employed. Slicing
of ovaries is a simple and efficient tool for recovering
good quality oocytes, but the aspiration technique is
laborious and time consuming. These reports also
confirm the report of Pawshe et al. (1994). The
grade A and B oocytes were retrieved greatly from
aspiration technique and dissection technique when
compared to grade C and D oocytes; the latter were
recovered to a greater extent by slicing method.
These findings were in confirmation with the report
of Gogoi et al. (2001).
From the results of the present study, the maximum
per cent yield of grade A and B oocytes was
observed by aspiration technique and dissection
technique, while the grade C and D oocytes were
retrieved by slicing technique. Overall yield of
oocytes was highest with slicing technique when
compared to the other techniques.
2 Materials and Methods
The present study was undertaken during February to
October months on local goat breeds that were
maintained by the shepherd of Hyderabad, Andhra
Pradesh. A total number of 120 non-gravid genitalia of
local does were collected immediately after slaughter
at municipal slaughter house and transported to the
laboratory within 2 hr in a thermos at 34
℃
in normal