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International Journal of Molecular Veterinary Research
2013, Vol.3, No.2, 4
-
6
http://ijmvr.sophiapublisher.com
4
Research Report
Open Access
Oocyte Retrieval Methods, Grade and Percentage of Oocytes in Goats
L. Ram Singh
1
, K. Sadasiva Rao
2
, K. Murali Mohan
3
1. Assistant Professor, Department of Animal Reproduction, Gyanecology and Obstetrics
2. Professor & Univ. Head, Dept. of VGO, CVSc, Rajendranagar, Hyderabad- 500 030 (A.P.) INDIA
3. Assistant Professor, Dept. of VGO, CVSc, Rajendranagar, Hyderabad- 500 030 (A.P.) INDIA
Corresponding author email: ramsinghl@yahoo.com;
Authors
International Journal of Molecular Veterinary Research, 2013, Vol.3, No.2 doi: 10.5376/ijmvr.2013.03.0002
Received: 15 Feb., 2013
Accepted: 04 Mar., 2013
Published: 21 Mar., 2013
Copyright
© 2013 Singh et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Singh et al., 2013, Oocyte Retrieval Methods, Grade and Percentage of Oocytes in Goats, International Journal of Molecular Veterinary Research, Vol.3, No.2
4-6 (doi: 10.5376/ ijmvr.2013.03.0002)
Abstract
The present study was undertaken to assess the relative efficiency of three different collection techniques of percentage
and grades of oocytes in goats of Hyderabad. The mean oocyte recovery rate from aspiration technique, 2.15 ± 0.31 and 2.9 ± 0.46 in
dissection technique and 6.55 ± 0.57 and 4.51 ± 0.46 in slicing technique. The mean oocyte recovery rate was 1.5 ± 0.22 and 1.0 ±
0.33 under aspiration technique, 2.58 ± 0.47 and 2.43 ± 0.36 under dissection technique and 5.26 ± 0.46 and 6.3 ± 0.71. The mean
numbers of oocytes of different grades recovered were 0.34 ± 0.03, 0.62 ± 0.07 and 1.37 ± 0.09 in aspiration, dissection and slicing
techniques, respectively. Among the different grades of oocytes, significantly higher per cent of A (27.78) and B (38.89) grade
oocytes were retrieved by aspiration and dissection technique when compared to slicing technique. With respect to C and D grade
oocytes, significantly higher numbers of oocytes were retrieved by slicing technique than the aspiration and dissection techniques.
Out of 437 oocytes retrieved in the present study, significantly higher per cent (50.57) yield was by slicing technique, followed by
dissection technique (37.07) and aspiration (12.53).
Keywords
Oocyte grading; Percentage; Aspiration; Dissection; Slicing; Goat
Introduction
Reproduction is the basic field of live stock
production. The genetic improvement of live stock can
be obtained by maximum utilization of proven sires
and dams by following the artificial insemination and
Embryo Transfer Technology (ETT). Follicular
oocytes could be matured
in vitro
and used for
in
vitro
fertilization for producing large number of
embryos (Agarawal, 1992; Suzuki et al., 1992).
In
vitro
techniques are powerful tools for studying
physiology of maturation, fertilization, development
of pre-implantation embryos and increasing
production as it gives access to micromanipulation of
embryos. For such studies, a large number of
in vitro
produced embryos are needed, which in turn need
larger number of oocytes only. The total number of
oocytes obtained per ovary is varied with different
techniques. Therefore, for efficient
in vitro
production
of embryo from ovaries procured from abattoirs is
necessary to develop technique that can maximize the
oocyte recovery. In case of the goat, the first birth
using the IVF procedure on ovulated oocytes was
achieved by Hanada (1985) and in the last few years,
many studies on
in vitro
maturation and IVF of goat
oocytes were conducted.
1 Results and Discussion
1.1 Oocyte retrieval
The number of oocytes retrived was significantly
higher in slicing method (5.52 ± 0.40), followed by
dissection (2.52 ± 0.28) and aspiration (1.35 ± 0.18)
(Figure 1). Similar techniques were also employed
by Wani et al. (2000), Kumar et al. (2000), Gogoi et
al. (2001) and Shirazi et al. (2005) with variable
number or per cent of oocytes retrived in the ovaries
of small ruminants.
The different A, B, C and D grades of oocytes
retrieved from left and right ovaries under aspiration,
dissection and slicing techniques were not
significantly differed between the numbers of
oocytes except in B grade oocyte, significantly