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Plant Gene and Trait, 2013, Vol.4, No.5, 25
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26
the physiological efficiency including the photosynthetic
ability of crop and play a significant role in improving
the productive potential of the crop. With this above
background, the present investigation was carried out.
2 Materials and methods
The present investigation was undertaken under field
condition to study the effect of nutrients and plant
growth regulators on growth and productivity of black
gram variety CO 5. The research experiment was carried
out at Millet Breeding Station, Tamil Nadu Agricultural
University, Coimbatore during July to October, 2007.
Growth regulators like Naphthalene Acetic Acid (NAA),
Salicylic Acid (SA), Cycocel (CCC), Brassinosteriod
(BR), Humic Acid and the nutrients such as Nitrogen,
DAP, Boric Acid, Ferrous Sulphate, Zinc Sulphate
were used. The data were statistically analyzed with
the Design of Randomized Block Design with three
replication and the Plot size of 4 m × 3 m with Spacing
of 30 cm 10 cm. in this research study having nine
treatments and the details are, T
1
- Control , T
2
- N 25
Kg/ha + Urea 2 % + NAA 40 ppm, T
3
- N 50 Kg/ha +
CCC 200 ppm, T
4
- N 25 kg / ha + Urea 2 % + CCC 200
ppm, T
5
- N 25 kg/ha + Urea 2 % + Humic acid 0.1 %,
T
6
- N 25 kg/ha + Urea 2 % + Salicylic acid 100 ppm,
T
7
- N 25 kg/ha + Urea 2 % + Brassinosteriod 0.1 ppm,
T
8
- N 25 kg/ha + Urea 2 % + ZnSO
4
0.5% + FeSO
4
0.5
+ Borax 0.2%, T
9
- N 25 kg/ha + Water spray.
Chlorophyll a, b and total chlorophyll were estimated in
physiologically active (index) leaf as per the proceture
of Yoshida et al. (1971) and expressed as mg/g
fresh
weight.
The content of soluble protein was estimated from the
leaf samples following the method of Lowry et al. (1951)
and expressed as mg/g fresh weight.
3 Results
3.1 Chlorophyll 'a' content (mg/g)
The data on chlorophyll ‘a’ (mg) content of black gram
variety CO 5 as influenced by the nutrients and plant
growth regulators at various growth stages is presented
in Table 1. In general, the chlorophyll 'a' content was
found to be maximum at the pod filling stage (60 DAS),
with a decline towards the harvest stage. The treatment
T
7
(N 25 kg/ha+ urea 2% + 0.1 ppm BR) recorded the
maximum chlorophyll ‘a’ content of 0.96 mg, 1.26 mg,
1.84 mg and 1.45 mg at vegetative, flowering, pod
filling and harvest stages, respectively.
3.2 Chlorophyll 'b' content (mg/g)
Foliar spraying of nutrients along with growth regulators
had significant effect on the chlorophyll 'b' content values
at all the stages of crop growth (Table 2). The results
Table 1 Effect of nitrogen nutrition and growth regulators on Chlorophyll ‘a’ content (mg/g) in black gram at different growth stages
Treatments
30 DAS
45 DAS
60 DAS
Harvest Stage
T
1
0.31
0.50
1.00
0.51
T
2
0.56
0.71
1.26
0.89
T
3
0.69
1.03
1.42
1.12
T
4
0.83
1.11
1.62
1.34
T
5
0.42
0.59
1.13
0.71
T
6
0.54
0.65
1.17
0.82
T
7
0.96
1.26
1.84
1.45
T
8
0.43
0.53
1.08
0.88
T
9
0.41
0.49
1.16
0.79
Mean
0.54
0.73
1.29
0.94
SEd
0.02
0.03
0.05
0.03
CD (0.05)
0.04
0.06
0.11
0.08
Note: T
1
: Control; T
2
: N 25 kg/ha +Urea 2 % + NAA 40 ppm; T
3
: N 50 kg/ha + CCC 200 ppm;T
4
: N 25 kg/ha +Urea 2 % + CCC 200
ppm; T
5
: N 25 kg/ha + Urea 2% + Humic acid 0.1%; T
6
: N 25 kg/ha + Urea 2 % + Salicylic acid 100 ppm; T
7
: N 25 kg/ha + Urea 2 % +
Brassinosteriod 0.1 ppm; T
8
: N 25 kg/ha + Urea 2 % + ZnSO
4
0.5% + FeSO
4
0.5% + Borax 0.2%; T
9
: N 25 kg/ha + Water spray