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Plant Gene and Trait, 2013, Vol.4, No.5, 25
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Table 2 Effect of nitrogen nutrition and growth regulators on Chlorophyll ‘b’ content (mg/g) in black gram at different growth stages
Note: T
1
: Control ; T
2
: N 25 Kg/ha +Urea 2 % + NAA40 ppm;T
3
: N 50 Kg/ha + CCC 200 ppm;T
4
: N 25 Kg/ha +Urea 2 % + CCC 200
ppm;T
5
: N 25 Kg/ha + Urea 2% + Humic acid 0.1%; T
6
: N 25 Kg/ha + Urea 2 % + Salicylic Acid 100ppm; T
7
: N 25 Kg/ha + Urea 2 % +
Brassinosteriod 0.1 ppm; T
8
: N 25 Kg/ha + Urea 2 % + ZnSO
4
0.5% + FeSO
4
0.5% + Borax 0.2%; T
9
: N 25 Kg/ha + Water spray
indicated an increase in chlorophyll 'b' content from
vegetative (30 DAS) to pod filling stage (60 DAS), which
showed a decline trend at harvest stage. The values
ranged from 0.29 mg/g~0.82
mg/g, 0.40 mg/g~0.94
mg/g, 0.48 mg/g~1.04 mg/g and 0.38 mg/g~0.99 mg/g
at vegetative, flowering, pod filling and harvest stages,
respectively. The effect of brassinolide was very evident
from the high values recorded in this treatment T
7
(N 25
kg/ha + urea 2% + 0.1 ppmBR) at all the stages of growth.
The control treatment recorded significantly lower values
(0.29 mg/g, 0.40 mg/g, 0.48 mg/g and 0.38 mg/g).
3.3 Soluble protein (mg/g)
The data on soluble protein content (mg) of leaves was
recorded at various stages of crop growth
viz.,
vegetative
(30 DAS), flowering (45 DAS), pod filling (60 DAS) and
harvest stage (Table 3). The soluble protein content
increased up to pod filling stage (60 DAS) with a decline
at harvest stage, which ranged from 12.8 to 15.4 mg/g,
15.2 to 19.5 mg/g, 19.1 to 26.7 mg/g and 10.0 mg/g ~
16.4 mg/g, respectively. The treatment T
7
(N 25 kg/ha +
urea 2% + 0.1 ppm BR) registered more soluble protein
content of 15.4 mg/g, 19.5 mg/g, 26.7 mg/g and 16.4
mg/g at all the stages of crop growth.
Table 3 Effect of nitrogen nutrition and growth regulators on soluble protein content (mg/g) in black gram at different growth stages
Note: T
1
: Control ; T
2
: N 25 Kg/ha +Urea 2 % + NAA 40 ppm;T
3
: N 50 kg/ha + CCC 200 ppm;T
4
: N 25 kg/ha +Urea 2 % + CCC 200
ppm;T
5
: N 25 kg/ha + Urea 2 % + Humic acid 0.1%; T
6
: N 25 kg/ha + Urea 2 % + Salicylic Acid 100 ppm; T
7
: N 25 kg/ha + Urea 2 % +
Brassinosteriod 0.1 ppm; T
8
: N 25 kg/ha + Urea 2 % + ZnSO
4
0.5% + FeSO
4
0.5% + Borax 0.2%; T
9
: N 25 kg/ha + Water spray
Treatments
30 DAS
45 DAS
60 DAS
Harvest stage
T
1
0.29
0.40
0.48
0.38
T
2
0.50
0.71
0.74
0.64
T
3
0.68
0.88
0.97
0.87
T
4
0.75
0.91
1.02
0.94
T
5
0.38
0.59
0.61
0.60
T
6
0.47
0.65
0.68
0.59
T
7
0.82
0.94
1.04
0.99
T
8
0.34
0.48
0.55
0.51
T
9
0.31
0.46
0.52
0.46
Mean
0.54
0.68
0.73
0.66
SEd
0.02
0.03
0.03
0.02
CD (0.05)
0.04
0.06
0.06
0.06
Treatments
30 DAS
45 DAS
60 DAS
Harvest stage
T
1
12.8
15.2
19.1
10.0
T
2
13.7
16.9
22.5
12.6
T
3
14.8
18.2
23.9
14.9
T
4
14.5
18.1
23.8
14.8
T
5
13.2
16.6
22.3
12.5
T
6
13.0
15.9
20.1
11.6
T
7
15.4
19.5
26.7
16.4
T
8
14.1
17.2
23.0
13.9
T
9
12.9
15.7
19.8
11.0
Mean
13.80
17.00
22.32
13.06
SEd
0.58
0.71
0.93
0.54
CD (0.05)
1.23
1.51
1.98
1.15