Molecular Plant Breeding 2016, Vol.7, No.23, 1
-
9
8
Polymorphism information content
(PIC
i
) = 1 −∑P
ij
j=1
where, P
ij
is the frequency of the j
th
allele for the i
th
marker, and summed over ‘n’ alleles.
The arithmetic mean heterozygosity for a marker (Hav) was calculated by Hav = Hn /n [n= number of markers on
loci analyzed (Powell et al., 1996). Heterozygosity for a marker (Hn) = 1-pi
2
, where pi is the allele frequency of i
th
allele (Nei et al., 1979). The average heterozygosity for polymorphic markers was derived by (Hav) p= δHn /np
( np= no. of polymorphic markers or loci). Marker Index was calculated as the product of two functions: DI
(Diversity index) and EMR (Effective Multiplex Ratio). DI of a primer is defined as 1-pi
2
, where pi is the
frequency of the ith allele (band). EMR of a primer is defined as ‘‘the product of the fraction of polymorphic loci
and the number of polymorphic loci for an individual assay’’ (Prevost and Wilkinson, 1999).
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