Molecular Plant Breeding 2016, Vol.7, No.23, 1
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9
6
In the present study, genomic diversity was studied in twenty-five lentil genotypes (Indian/Exotic genotypes and
collection). Out of 100 ISSR markers tested, 24 produced unambiguous amplicons. Amplified markers produced
easily scorable bands ranging from 160 to 3000 bp in length. A total of 156 ISSR fragments were amplified with
an average of 6.5 amplicons per marker. The most informative markers were selected on the basis of extent band
polymorphism, detected by the individual marker. Also, 11 unique bands were found only in particular genotypes.
A 900 bp band was obtained in genotype DPL-44 with marker UBC-808. Similarly the marker UBC-815
amplified two unique bands of 1120 bp and 400 bp in case of Sehore74-3 and IPL525.
Figure 3 Two dimensional scaled plot of Lentil cultivars
Figure 4 Three dimensional scaled plot of Lentil cultivars
I
-1.44
-0.80
-0.16
0.48
1.12
II
-1.20
-0.69
-0.19
0.32
0.82
IPL525
PRECOZ
DPL15
WBL58
SEHORE
PL234
PL5
K75
L4076
PL406
L-912
IPL81
NDL1
LH84-8
JL1
LL147
L4147
VL103
T-36
LH82-6
DPL62
ILL6002
ILL8114
P2016
DPL44
DPL44
P2016
ILL8114
ILL6002
DPL62
LH82-6
T-36
VL103
L4147
LL147
JL1
LH84-8
NDL1
IPL81
L-912
PL406
L4076
K75
PL5
PL234
SEHORE
WBL58
DPL15
PRECOZ
IPL525
