Molecular Plant Breeding 2016, Vol.7, No.23, 1
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might be due to their origin and adaptation from same place Jabalpur, in India. Morphologically also they are
similar to each other in relation to foliage colour, size and hairiness. The maximum distance was found between
genotypeT-36 and Precoz which is also evident by their genetic similarity (37%), which is lowest among all pair
of genotypes. The next distant pairwas IPL525 and T36, with genetic similarity of 40.9%. The average similarity
was found to be 69.32% among all genotypes based on ISSR marker data.
The genotype LL147 and L4147 were appears closely in three dimensional plot which proves that they are highly
similar which is also clear by their high genetic similarity value (84%) followed by L4147 and VL103 (82.9%)
(Figure 3; Figure 4). The maximum distance appears between T-36 and Precoz (37.0%) which was followed by
IPL525 and T36 (40.9%). The similar kind of result was obtained in two-dimensional analysis also. The average
similarity was found to be 69.32% among all the genotypes based on ISSR data, which indicated relatively narrow
genetic base of lentil.
2 Discussion
Genetic variations in crop plants has continued to narrow down due to continuous selection pressure for specific
traits i.e. yield, thus rendering them more vulnerable to disease and insect epidemics and jeopardizing the
potential for long term sustained genetic improvement.
Therefore, it is extremely important to study the genetic relationship of the existing modern-day genotypes in
comparison with their ancestors and related species. This will not only provide information on their genetic
distance and phylogenetic relationship but will also indicate a chance of finding new and useful genes.
Figure 2 Dendrogram of 25 lentil cultivars depicting genetic relationship derived from UPGMA cluster analysis using the Jaccard’s
similarity coefficients based on 156 amplicons from 24 ISSR markers
