International Journal of Horticulture, 2015, Vol.5, No.21, 1-45
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daylily species which, with a few minor exceptions, were generally supported by the AFLP data.
Taiwan lily (
Lilium longiflorum
Thunb. var. formosanum Baker) is distributed from lowlands to high mountains in
Taiwan with large morphological variation. The genetic differentiation of seven populations from low, middle, and
high altitudes was studied by evaluating seven morphological traits and 64 RAPD markers (Wen and Hsiao, 2001).
RAPD analysis employing nine primers also revealed that the populations were differentiated according to the
altitudinal differences. Result indicated that populations of high altitudes were more variable among individuals
within populations than were populations of low altitudes. Therefore, there is a need for immediate measures to
conserve the germplasms of lower altitude populations.
Attempts were made to develop molecular DNA markers for identification of hybrids of lily (Wiejacha et al.,
2001). Plants were obtained by ovule rescue from pollinations of lily cultivars belonging to the group "oriental
hybrids" with pollen of
Lilium henryi, L. pumilum
and
L.xformolongi
. The products of amplification were
detected for all the forty primers used. Eight primers generated reproducible polymorphic bands within the pair
'Marco Polo' and
L. henryi
, six primers within 'Alma Ata' and
L. pumilum
, and 'Muscadet' and
L.xformolongi
.
They found that some bands could serve as markers to detect hybrids at the
in vitro
stage.
Limonium
(fam.
Plumbaginaceae
) is grown in several regions of the world for use as a cut flower for both fresh
and dry-flower arrangements. In this work, thirteen wild species were tested for the study of genetic
relationships and taxonomic status using RAPD analyses. The dendrogram obtained from cluster analysis showed
high similarity among three species that some authors report as synonymous (
L. caspia
,
L. bellidifolium
and
L.
otolepis
). In order to clarify the genetic relationships, further analyses were carried out on several genotypes
belonging to these species. The new dendrogram showed that the genotypes did not group in clear clusters.
Analysis of molecular variance (AMOVA) confirmed that the species can be considered synonymous. The use of
RAPD markers was thus useful for clarifying the highly probable identity of the three
Limonium
species, in a
plant genus that is notably of difficult interpretation (Bruna et al., 2004).
RAPD was used for genetic fingerprinting of
Limonium
spp.,
Alstroemeria
spp., carnation and
Prunus
spp. for
verification of hybridity in progenies of interspecific crosses and for the study of taxonomic relationships. The
putative percentage was confirmed in 4 hybrids and it was excluded in one hybrid showing both a completely
different phenotype and RAPD patterns from its putative parents. RAPD markers were also used for the
characterization of potential parents in a breeding programme on
Alstroemeria
for pot plant production. In
carnation, RAPD analysis was used for the identification of molecular markers associated with cut flower
longevity. Regression analysis showed a positive correlation between the score of each progeny (number of
RAPD markers similar to Roland) and its longevity (Chung et al., 2002).
Mor et al. (2008) used nine genotypes of marigold, selected from two species,
Tagetes erecta
L. (3 genotypes) and
Tagetes patula
L. (6 genotypes) and characterized through electrophoresis of protein and using RAPD markers.
The dendrogram based on protein electrophoresis grouped the nine genotypes in two clusters species -wise,
whereas RAPD analysis showed clear-cut genotype and species difference, which confirms the reliability of
RAPD markers over protein electrophoresis.
Myrtus communis
L., is a wild shrub widespread throughout the Mediterranean region and the Middle East, grown
for its ornamental value and aromatic properties. Bruna et al. (2007) evaluated the genetic diversity existing
within wild myrtle populations for the development of breeding programs or selection of genotypes with useful
traits for cultivar improvement using AFLP markers. Fifty-one individuals were analysed with three selected
combination of primers. Analysis of molecular variance revealed that variability within populations represented
the greatest source of variation.
Guo et al. (2007) used RAPD markers to estimate the genetic diversity and to test the genetic basis of the
relationships between morphotypes and molecular markers of 65 lotus accessions in genus
Nelumbo.
Neither the
