International Journal of Horticulture 2015, Vol.5, No.3, 1
-
12
6
Instrumental conditions
Instrument
HPLC with DAD (Agilent 1200 series)
Mobile phase
Methanol: water (65:35 v/v)
Flow rate
0.5 ml min
-1
Wave length (nm)
230
Injection volume
20 µl (using auto sampler)
Temperature
Ambient
Column
Eclipse XDB C
18
(5cm, 4.6 x 150 mm)
Retention time
6.25 min (±0.2 min)
Extraction and analysis of oxyfluorfen
50 g of turmeric rhizome (after cutting into very small
pieces) from each replication was taken in a
homogenizer and was grinded into small pieces.
Similarly the soil samples were homogenized and 50 g
was weighed. Oxyfluorfen residue was extracted using
acetone by shaking for two hours in a reciprocating
shaker and the extract was filtered and evaporated at
40
o
C using rotary vaccum evaporator to about 10 ml.
The concentrated extract was transferred to a glass
column filled with anhydrous sodium sulfate and
activated charcoal and eluted with 100 ml of acetone.
Eluted lower layer was concentrated on rotary vacuum
evaporator at 60
o
C to moistened level and volume was
made to approximately 2 ml using acetone for GC
analysis.
Instrumental conditions
Detector
Electron capture detector
Column
Megabore capillary column D13-30 m
×
0.53 mm ID-BPL0.5 µm
Injector
240
o
C
Column
185-190
o
C
Detector
260
o
C
Carrier gas flow rate
30 ml min
-1
Retention time
4.98 min
Extraction and analysis of oxadiargyl
The representative turmeric rhizome (5 g) and soil (10 g)
samples were weighed and blended separately. The
samples were transferred to a 50 ml centrifuge tube and
added with 4 g MgSO
4
, 1 g NaCl and 15 ml acetonitrile.
Immediately the sample mixture was vigorously shaken
for 1 min and then centrifuged at 2000 RPM for 5 min.
A portion (5 ml) of the supernatant was transferred to a
flask and concentrated to near dryness then blowed to
dry with rotary vacuum evaporator at 60°C to
moistened level. The residue was re-dissolved in ethyl
acetate for GC injection.
[min.]
Time
0
2
4
6
8
10
12
14
[V]
Voltage
0.0
0.2
0.4
0.6
0.8
Oxyfluorfen
C:\IRIS32\WORK1\DATA\2013\TURMERIC\0.5 PPM OXY
4.980
